Scribble (Phospho Ser1220) rabbit pAb
- 货号：YP1479
- 应用：WB;ELISA;IHC
- 种属：Human;Mouse
- 简介：
- >>Hippo signaling pathway;>>Tight junction;>>Human papillomavirus infection;>>Viral carcinogenesis
- 基因名称：
- SCRIB CRIB1 KIAA0147 LAP4 SCRB1 VARTUL
- 免疫原：
- Synthesized phosho peptide around human Scribble (Ser1220)
- 特异性：
- This antibody detects endogenous levels of Human Mouse Scribble (phospho-Ser1220)
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- 纯化工艺：
- The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- Protein scribble homolog (Scribble) (hScrib) (Protein LAP4)
- 背景：
- This gene encodes a protein that was identified as being similar to the Drosophila scribble protein. The mammalian protein is involved in tumor suppression pathways. As a scaffold protein involved in cell polarization processes, this protein binds to many other proteins. The encoded protein binds to papillomavirus E6 protein via its PDZ domain and the C-terminus of E6. Two alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Nov 2011],
- 功能：
- similarity:Contains 4 PDZ (DHR) domains.,
- 细胞定位：
- Cell membrane ; Peripheral membrane protein. Cell junction . Cell junction, adherens junction. Cell projection, lamellipodium. Cytoplasm. Cell junction, synapse, postsynapse . Cell junction, synapse, presynapse . Targeting to cell-cell junctions which is CDH1-dependent is required for the pro-apoptotic activity. In a subset of CD4+ T-cells, colocalizes with CRTAM at the immunological synapse during the late phase of T-cell activation (By similarity). .
- 组织表达：
- Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level).
- Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
