Rad18 (Phospho Ser403) rabbit pAb

    • 货号:YP1458
    • 应用:WB;ELISA;IHC
    • 种属:Human;Rat;Mouse;
      • 靶点:
      • Rad18
      • 基因名称:
      • RAD18 RNF73
      • 蛋白名称:
      • Rad18 (Ser403)
      • Human Swiss Prot No:
      • Q9NS91
      • Mouse Swiss Prot No:
      • Q9QXK2
      • 免疫原:
      • Synthesized phosho peptide around human Rad18 (Ser403)
      • 特异性:
      • This antibody detects endogenous levels of Human Rad18 (phospho-Ser403)
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • E3 ubiquitin-protein ligase RAD18 (EC 6.3.2.-) (Postreplication repair protein RAD18) (hHR18) (hRAD18) (RING finger protein 73)
      • 实测条带:
      • 56kD
      • 背景:
      • The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq, Jul 2008],
      • 功能:
      • function:E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.,pathway:Protein modification; protein ubiquitination.,similarity:Belongs to the RAD18 family.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 1 SAP domain.,subunit:Interacts with UBE2A and UBE2B. Interacts with SHPRH.,
      • 细胞定位:
      • Nucleus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Associates with chromatin (PubMed:25931565). Colocalizes with SLF1 in the nucleus and to centrosomes (PubMed:15632077). Relocalizes with SLF1 to nuclear foci in response to DNA damage (PubMed:22036607). Accumulates with the SLF1-SLF2 and SMC5-SMC6 complexes at replication-coupled DNA interstrand repair and DNA double-strand breaks (DSBs) sites on chromatin in a ubiquitin-dependent manner (PubMed:25931565). .
      • 组织表达:
      • Epithelium,Liver,Placenta,
      • 产品图片
      • Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).