- 靶点:
- MLKL
- 简介:
- >>Necroptosis;>>TNF signaling pathway;>>Salmonella infection
- 基因名称:
- MLKL
- 蛋白名称:
- MLKL (phospho S358)
- Human Gene Id:
- 197259
- Human Swiss Prot No:
- Q8NB16
- 免疫原:
- Synthesized peptide derived from human MLKL (phospho S358)
- 特异性:
- This antibody detects endogenous phospho levels of MLKL (phospho S358) at Human, Mouse, Rat
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- IHC 1:100-500;IF ICC 1:100-500
- 纯化工艺:
- The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
- 浓度:
- 1 mg/ml
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- Mixed lineage kinase domain-like protein
- 实测条带:
- 55kD
- 背景:
- This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015],
- 功能:
- domain:The protein kinase domain is predicted to be catalytically inactive.,similarity:Belongs to the protein kinase superfamily.,similarity:Contains 1 protein kinase domain.,
- 细胞定位:
- Cytoplasm . Cell membrane . Nucleus . Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction (PubMed:24316671). Localizes to the nucleus in response to orthomyxoviruses infection (By similarity). .
- 组织表达:
- Chondrocyte,Leukocyte,Lymph node,
IMB5036 inhibits human pancreatic cancer growth primarily through activating necroptosis. BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY Basic Clin Pharmacol. 2022 Mar;130(3):375-384 IF Human Capan-2 cell,BxPC-3 cell
货号:YP1244
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- Antibody-FAQs
- 产品图片
- Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1,primary Antibody was diluted at 1:200(4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200