DAPK2 (phospho Ser318) Polyclonal Antibody
- 货号:YP1142
- 应用:IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 靶点:
- DAPK2
- 简介:
- >>Autophagy - animal;>>Pathways in cancer;>>Bladder cancer
- 基因名称:
- DAPK2
- 蛋白名称:
- Death-associated protein kinase 2
- Human Gene Id:
- 23604
- Human Swiss Prot No:
- Q9UIK4
- Mouse Gene Id:
- 13143
- Mouse Swiss Prot No:
- Q8VDF3
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human DAPK2 around the phosphorylation site of Ser318. AA range:284-333
- 特异性:
- Phospho-DAPK2 (S318) Polyclonal Antibody detects endogenous levels of DAPK2 protein only when phosphorylated at S318.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 浓度:
- 1 mg/ml
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- DAPK2;Death-associated protein kinase 2;DAP kinase 2;DAP-kinase-related protein 1;DRP-1
- 分子量:
- 43kD
- 背景:
- This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. [provided by RefSeq, Jul 2008],
- 功能:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Negatively regulated by autophosphorylation on Ser-318.,function:Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Homodimer. Homodimerization is required for apoptotic function and is inhibited by autophosphorylation at Ser-318.,tissue specificity:Ubiquitously expressed in all tissue types examined. High levels in heart, lung and skeletal muscle.,
- 细胞定位:
- Cytoplasm. Cytoplasmic vesicle, autophagosome lumen.
- 组织表达:
- Expressed in neutrophils and eosinophils (PubMed:24163421). Isoform 2 is expressed in embryonic stem cells (at protein level). Isoform 1 is ubiquitously expressed in all tissue types examined with high levels in heart, lung and skeletal muscle.
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- 产品图片
- Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using DAPK2 (Phospho-Ser318) Antibody
- Immunofluorescence analysis of COS7 cells, using DAPK2 (Phospho-Ser318) Antibody. The picture on the right is blocked with the phospho peptide.
- Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using DAPK2 (Phospho-Ser318) Antibody. The picture on the right is blocked with the phospho peptide.