• 靶点：
• DAPK1

• 简介：
• >>Autophagy - animal;>>Pathways in cancer;>>Bladder cancer

• 基因名称：
• DAPK1

• 蛋白名称：
• Death-associated protein kinase 1

• Human Gene Id：
• 1612

• Human Swiss Prot No：
• P53355

• Mouse Gene Id：
• 69635

• Mouse Swiss Prot No：
• Q80YE7

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human DAPK1 around the phosphorylation site of Ser308. AA range:274-323

• 特异性：
• Phospho-DAPK1 (S308) Polyclonal Antibody detects endogenous levels of DAPK1 protein only when phosphorylated at S308.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• DAPK1;DAPK;Death-associated protein kinase 1;DAP kinase 1

• 分子量：
• 160kD

• 背景：
• Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013],

• 功能：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Negatively regulated by autophosphorylation on Ser-308.,function:Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.,induction:Up-regulated following treatment with interferon-gamma.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.,similarity:Contains 1 death domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 10 ANK repeats.,subcellular location:Colocalizes with the actin filament system.,

• 细胞定位：
• [Isoform 1]: Cytoplasm. Cytoplasm, cytoskeleton. Colocalizes with MAP1B in the microtubules and cortical actin fibers.; [Isoform 2]: Cytoplasm. Cytoplasm, cytoskeleton.

• 组织表达：
• Isoform 2 is expressed in normal intestinal tissue as well as in colorectal carcinomas.

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