APLF (phospho Ser116) Polyclonal Antibody
- 货号:YP1078
- 应用:IHC;IF;ELISA
- 种属:Human;Mouse
- 蛋白名称:
- Aprataxin and PNK-like factor
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human APLF around the phosphorylation site of Ser116. AA range:82-131
- 特异性:
- Phospho-APLF (S116) Polyclonal Antibody detects endogenous levels of APLF protein only when phosphorylated at S116.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- APLF;C2orf13;PALF;XIP1;Aprataxin and PNK-like factor;Apurinic-apyrimidinic endonuclease APLF;PNK and APTX-like FHA domain-containing protein;XRCC1-interacting protein 1
- 背景:
- C2ORF13 is a component of the cellular response to chromosomal DNA single- and double-strand breaks (Iles et al., 2007 [PubMed 17353262]).[supplied by OMIM, Mar 2008],
- 功能:
- domain:The FHA-like domain mediates interaction with XRCC1 and XRCC4.,function:Involved in single-strand and double-strand DNA break repair.,PTM:Phosphorylated in an ATM-dependent manner upon double-strand DNA break.,similarity:Contains 1 FHA-like domain.,similarity:Contains 2 C2H2-type zinc fingers.,subcellular location:Colocalizes with XRCC1 at sites of DNA damage.,subunit:Interacts with XRCC1. May also interact with XRCC4 and XRCC5.,
- 细胞定位:
- Nucleus . Chromosome . Cytoplasm, cytosol . Localizes to DNA damage sites (PubMed:18474613, PubMed:18172500, PubMed:21211721, PubMed:23689425). Accumulates at single-strand breaks and double-strand breaks via the PBZ-type zinc fingers (PubMed:18172500). .
- Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.