GCN2 (phospho Thr899) Polyclonal Antibody
- 货号：YP1042
- 应用：IHC;IF;ELISA
- 种属：Human;Mouse
- 简介：
- >>Autophagy - animal;>>Protein processing in endoplasmic reticulum;>>Hepatitis C;>>Measles;>>Herpes simplex virus 1 infection
- 蛋白名称：
- Eukaryotic translation initiation factor 2-alpha kinase 4
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human GCN2 around the phosphorylation site of Thr899. AA range:865-914
- 特异性：
- Phospho-GCN2 (T899) Polyclonal Antibody detects endogenous levels of GCN2 protein only when phosphorylated at T899.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- EIF2AK4;GCN2;KIAA1338;Eukaryotic translation initiation factor 2-alpha kinase 4;GCN2-like protein
- 背景：
- This gene encodes a member of a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2), resulting in the downregulaton of protein synthesis. The encoded protein responds to amino acid deprivation by binding uncharged transfer RNAs. It may also be activated by glucose deprivation and viral infection. Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2. [provided by RefSeq, Mar 2014],
- 功能：
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:Kinase domain 1 is a degenerate kinase domain.,domain:RWD domain is reported to interact with GCN1L1.,function:Can phosphorylate the alpha subunit of EIF2 and may mediate translational control.,PTM:Autophosphorylated on threonine residues.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.,similarity:Contains 1 RWD domain.,similarity:Contains 2 protein kinase domains.,tissue specificity:Widely expressed.,
- 组织表达：
- Widely expressed (PubMed:10504407). Expressed in lung, smooth muscle cells and macrophages (PubMed:24292273).
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
