Rabphilin-3A (phospho Ser237) Polyclonal Antibody

    • 货号:YP0891
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse;Rat
      • 靶点:
      • Rabphilin 3A
      • 基因名称:
      • RPH3A
      • 蛋白名称:
      • Rabphilin-3A
      • Human Swiss Prot No:
      • Q9Y2J0
      • Mouse Swiss Prot No:
      • P47708
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human Rabphilin 3A around the phosphorylation site of Ser237. AA range:203-252
      • 特异性:
      • Phospho-Rabphilin-3A (S237) Polyclonal Antibody detects endogenous levels of Rabphilin-3A protein only when phosphorylated at S237.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • RPH3A;KIAA0985;Rabphilin-3A;Exophilin-1
      • 实测条带:
      • 75kD
      • 背景:
      • Exocytosis of neurotransmitters and hormones is fundamental to synaptic neurotransmission and cell-cell communication. RAB3A (MIM 179390) is a small G protein that is thought to act at late stages of exocytosis, and RPH3A is a RAB3A effector (Lin et al., 2007 [PubMed 17149709]).[supplied by OMIM, Jul 2008],
      • 功能:
      • function:Protein transport. Probably involved with Ras-related protein Rab-3A in synaptic vesicle traffic and/or synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.,similarity:Contains 1 FYVE-type zinc finger.,similarity:Contains 1 RabBD (Rab-binding) domain.,similarity:Contains 2 C2 domains.,
      • 细胞定位:
      • Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane . Cell projection, dendritic spine . Cell junction, synapse, postsynaptic cell membrane . Membrane ; Peripheral membrane protein .
      • 组织表达:
      • Brain,Lung,
      • 产品图片
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Rabphilin 3A (Phospho-Ser237) Antibody
      • Immunofluorescence analysis of HeLa cells, using Rabphilin 3A (Phospho-Ser237) Antibody. The picture on the right is blocked with the phospho peptide.
      • Immunohistochemistry analysis of paraffin-embedded human brain, using Rabphilin 3A (Phospho-Ser237) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from RAW264.7 cells treated with Calyculin 100nM 15', using Rabphilin 3A (Phospho-Ser237) Antibody. The lane on the right is blocked with the phospho peptide.