p70 S6 kinase α (phospho Ser418) Polyclonal Antibody

    • 货号:YP0886
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse;Rat
      • 靶点:
      • p70 S6 kinase α
      • 简介:
      • >>EGFR tyrosine kinase inhibitor resistance;>>Endocrine resistance;>>ErbB signaling pathway;>>HIF-1 signaling pathway;>>Autophagy - animal;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>TGF-beta signaling pathway;>>Apelin signaling pathway;>>Fc gamma R-mediated phagocytosis;>>Thermogenesis;>>Insulin signaling pathway;>>Insulin resistance;>>Shigellosis;>>Human cytomegalovirus infection;>>Human papillomavirus infection;>>Human immunodeficiency virus 1 infection;>>Pathways in cancer;>>Proteoglycans in cancer;>>Chemical carcinogenesis - receptor activation;>>Colorectal cancer;>>Pancreatic cancer;>>Acute myeloid leukemia;>>Breast cancer;>>Hepatocellular carcinoma;>>Gastric cancer;>>Choline metabolism in cancer;>>PD-L1 expression and PD-1 checkpoint pathway in cancer
      • 基因名称:
      • RPS6KB1 STK14A P70S6K
      • 蛋白名称:
      • Ribosomal protein S6 kinase beta-1
      • Human Gene Id:
      • 6198
      • Human Swiss Prot No:
      • P23443
      • Mouse Swiss Prot No:
      • Q8BSK8
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human p70 S6 Kinase around the phosphorylation site of Ser418. AA range:384-433
      • 特异性:
      • Phospho-p70 S6 kinase α (S418) Polyclonal Antibody detects endogenous levels of p70 S6 kinase α protein only when phosphorylated at S418.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • RPS6KB1;STK14A;Ribosomal protein S6 kinase beta-1;S6K-beta-1;S6K1;70 kDa ribosomal protein S6 kinase 1;P70S6K1;p70-S6K 1;Ribosomal protein S6 kinase I;Serine/threonine-protein kinase 14A;p70 ribosomal S6 kinase alpha;p70 S6 kinas
      • 实测条带:
      • 60kD
      • 背景:
      • ribosomal protein S6 kinase B1(RPS6KB1) Homo sapiens This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013],
      • 功能:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activation by serine/threonine phosphorylation and protein kinase C, inactivated by type 2A phosphatase.,function:Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with PPP1R9A/neurabin-1.,tissue specificity:Widely expressed.,
      • 细胞定位:
      • Cell junction, synapse, synaptosome . Mitochondrion outer membrane. Mitochondrion. Colocalizes with URI1 at mitochondrion.; [Isoform Alpha I]: Nucleus. Cytoplasm.; [Isoform Alpha II]: Cytoplasm.
      • 组织表达:
      • Widely expressed.

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      RAPTOR promotes colorectal cancer proliferation by inducing mTORC1 and upregulating ribosome assembly factor URB1. Cancer Medicine Cancer Med-Us. 2020 Feb;9(4):1529-1543 WB,IHC Human 1:1500,1:200 colorectal cancer (CRC) tissue RKO cell, SW480 cell, HCT116 cell,LoVo cell
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      Fluoride regulates chondrocyte proliferation and autophagy via PI3K/AKT/mTOR signaling pathway. CHEMICO-BIOLOGICAL INTERACTIONS Chem-Biol Interact. 2021 Nov;349:109659 WB Mouse 1:1000 ATDC5 cells
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      • 产品图片
      • Wang, Tao, et al. "RAPTOR promotes colorectal cancer proliferation by inducing mTORC1 and upregulating ribosome assembly factor URB1." Cancer medicine 9.4 (2020): 1529-1543.
      • Western blot analysis of HT-29 serum 20% 15' using p-p70 S6 kinase α (S418) antibody. Antibody was diluted at 1:1000
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using p70 S6 Kinase (Phospho-Ser418) Antibody
      • Immunofluorescence analysis of COS7 cells, using p70 S6 Kinase (Phospho-Ser418) Antibody. The picture on the right is blocked with the phospho peptide.
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p70 S6 Kinase (Phospho-Ser418) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from HT29 cells treated with serum 20% 15', using p70 S6 Kinase (Phospho-Ser418) Antibody. The lane on the right is blocked with the phospho peptide.