c-Myc (phospho Ser62) Polyclonal Antibody

    • 货号:YP0861
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse;Rat
      • 靶点:
      • c-Myc
      • 简介:
      • >>MAPK signaling pathway;>>ErbB signaling pathway;>>Cell cycle;>>PI3K-Akt signaling pathway;>>Cellular senescence;>>Wnt signaling pathway;>>TGF-beta signaling pathway;>>Hippo signaling pathway;>>Signaling pathways regulating pluripotency of stem cells;>>JAK-STAT signaling pathway;>>Thyroid hormone signaling pathway;>>Salmonella infection;>>Hepatitis C;>>Hepatitis B;>>Human cytomegalovirus infection;>>Human T-cell leukemia virus 1 infection;>>Kaposi sarcoma-associated herpesvirus infection;>>Epstein-Barr virus infection;>>Pathways in cancer;>>Transcriptional misregulation in cancer;>>Proteoglycans in cancer;>>MicroRNAs in cancer;>>Chemical carcinogenesis - receptor activation;>>Colorectal cancer;>>Endometrial cancer;>>Thyroid cancer;>>Bladder cancer;>>Chronic myeloid leukemia;>>Acute myeloid leukemia;>>Small cell lung cancer;>>Breast cancer;>>Hepatocellular carcinoma;>>Gastric cancer;>>Central carbon metabolism in cancer
      • 基因名称:
      • MYC
      • 蛋白名称:
      • Myc proto-oncogene protein
      • Human Gene Id:
      • 4609
      • Human Swiss Prot No:
      • P01106
      • Mouse Swiss Prot No:
      • P01108
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human Myc around the phosphorylation site of Ser62. AA range:31-80
      • 特异性:
      • Phospho-c-Myc (S62) Polyclonal Antibody detects endogenous levels of c-Myc protein only when phosphorylated at S62.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • MYC;BHLHE39;Myc proto-oncogene protein;Class E basic helix-loop-helix protein 39;bHLHe39;Proto-oncogene c-Myc;Transcription factor p64
      • 实测条带:
      • 50,(also ~60KD in some samples)
      • 背景:
      • The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq, Jul 2008],
      • 功能:
      • disease:A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.,disease:Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.,function:Participates in the regulation of gene transcription. Binds DNA both in a non-specific manner and also specifically to recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.,online information:Myc entry,PTM:Phosphorylated by PRKDC.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with KDM5A and KDM5B.,
      • 细胞定位:
      • Nucleus, nucleoplasm . Nucleus, nucleolus .
      • 组织表达:
      • Cervix,Epithelium,Leukemia,Placenta,Promyelocytic l

      Tumor Suppressor DYRK1A Effects on Proliferation and Chemoresistance of AML Cells by Downregulating c-Myc. PLoS One 2014 Jun 05 WB Human HEL cell, HL-60 cell, NB4 cell
      货号:YP0861

      MPC1 Deficiency Promotes CRC Liver Metastasis via Facilitating Nuclear Translocation of β-Catenin. Journal of Immunology Research 2020 Aug 08 IHC Mouse,Human Liver
      货号:YP0861

      • 产品图片
      • Liu, Qiang, et al. "Tumor suppressor DYRK1A effects on proliferation and chemoresistance of AML cells by downregulating c-Myc." PloS one 9.6 (2014): e98853.
      • Western Blot analysis of various cells using Phospho-c-Myc (S62) Polyclonal Antibody diluted at 1:1000
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Myc (Phospho-Ser62) Antibody
      • Immunofluorescence analysis of HeLa cells treated with Forskolin 40nM 30', using Myc (Phospho-Ser62) Antibody. The picture on the right is blocked with the phospho peptide.
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Myc (Phospho-Ser62) Antibody. The picture on the right is blocked with the phospho peptide.