• 靶点：
• Topo IIα

• 简介：
• >>Platinum drug resistance

• 基因名称：
• TOP2A

• 蛋白名称：
• DNA topoisomerase 2-alpha

• Human Gene Id：
• 7153

• Human Swiss Prot No：
• P11388

• Mouse Gene Id：
• 21973

• Mouse Swiss Prot No：
• Q01320

• Rat Gene Id：
• 360243

• Rat Swiss Prot No：
• P41516

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human TOP2A around the phosphorylation site of Ser1525. AA range:1482-1531

• 特异性：
• Phospho-Topo IIα (S1525) Polyclonal Antibody detects endogenous levels of Topo IIα protein only when phosphorylated at S1525.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• TOP2A;TOP2;DNA topoisomerase 2-alpha;DNA topoisomerase II; alpha isozyme

• 实测条带：
• 190kD

• 背景：
• This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also pla

• 功能：
• catalytic activity:ATP-dependent breakage, passage and rejoining of double-stranded DNA.,enzyme regulation:Specifically inhibited by the intercalating agent amsacrine.,function:Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.,miscellaneous:Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.,PTM:Phosphorylation has no effect on catalytic activity.,similarity:Belongs to the type II topoisomerase family.,subcellular location:Generally located in the nucleoplasm.,subunit:Homodimer. Interacts with COPS5.,

• 细胞定位：
• Cytoplasm . Nucleus, nucleoplasm . Nucleus . Nucleus, nucleolus .

• 组织表达：
• Expressed in the tonsil, spleen, lymph node, thymus, skin, pancreas, testis, colon, kidney, liver, brain and lung (PubMed:9155056). Also found in high-grade lymphomas, squamous cell lung tumors and seminomas (PubMed:9155056).

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