• 靶点：
• Syntaxin 1

• 简介：
• >>SNARE interactions in vesicular transport;>>Synaptic vesicle cycle;>>Insulin secretion;>>Huntington disease;>>Pathways of neurodegeneration - multiple diseases;>>Amphetamine addiction

• 基因名称：
• STX1A

• 蛋白名称：
• Syntaxin-1A

• Human Gene Id：
• 6804

• Human Swiss Prot No：
• Q16623

• Mouse Gene Id：
• 20907

• Mouse Swiss Prot No：
• O35526

• Rat Gene Id：
• 116470

• Rat Swiss Prot No：
• P32851

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human Syntaxin 1A around the phosphorylation site of Ser14. AA range:1-50

• 特异性：
• Phospho-Syntaxin 1 (S14) Polyclonal Antibody detects endogenous levels of Syntaxin 1 protein only when phosphorylated at S14.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• STX1A;STX1;Syntaxin-1A;Neuron-specific antigen HPC-1

• 实测条带：
• 35kD

• 背景：
• This gene encodes a member of the syntaxin superfamily. Syntaxins are nervous system-specific proteins implicated in the docking of synaptic vesicles with the presynaptic plasma membrane. Syntaxins possess a single C-terminal transmembrane domain, a SNARE [Soluble NSF (N-ethylmaleimide-sensitive fusion protein)-Attachment protein REceptor] domain (known as H3), and an N-terminal regulatory domain (Habc). Syntaxins bind synaptotagmin in a calcium-dependent fashion and interact with voltage dependent calcium and potassium channels via the C-terminal H3 domain. This gene product is a key molecule in ion channel regulation and synaptic exocytosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009],

• 功能：
• disease:Haploinsufficiency of STX1A may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in Williams-Beuren syndrome (WBS), a rare developmental disorder. It is a contiguous gene deletion syndrome involving genes from chromosome band 7q11.23.,function:Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis.,similarity:Belongs to the syntaxin family.,similarity:Contains 1 t-SNARE coiled-coil homology domain.,subunit:Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds to CPLX1. Binds SYTL4 and STXBP6. Found in a ternary complex with STX1A and SNAP25. Interacts with OTOF (By similarity). Found in a complex with VAMP8 and SNAP23. Interacts with VAPA and SYBU.,tissue specificity:Isoform 1 is highly expressed in embryonic spinal chord and ganglia

• 细胞定位：
• Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane ; Single-pass type IV membrane protein . Cell junction, synapse, synaptosome . Cell membrane . Colocalizes with KCNB1 at the cell membrane. .; [Isoform 2]: Secreted .

• 组织表达：
• [Isoform 1]: Highly expressed in embryonic spinal cord and ganglia and in adult cerebellum and cerebral cortex. ; [Isoform 2]: Expressed in heart, liver, fat, skeletal muscle, kidney and brain.

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