• 靶点：
• PC-PLD1

• 简介：
• >>Glycerophospholipid metabolism;>>Ether lipid metabolism;>>Metabolic pathways;>>Ras signaling pathway;>>cAMP signaling pathway;>>Sphingolipid signaling pathway;>>Phospholipase D signaling pathway;>>Endocytosis;>>Fc gamma R-mediated phagocytosis;>>Glutamatergic synapse;>>GnRH signaling pathway;>>Parathyroid hormone synthesis, secretion and action;>>Pathways in cancer;>>Chemical carcinogenesis - reactive oxygen species;>>Pancreatic cancer;>>Choline metabolism in cancer

• 基因名称：
• PLD1

• 蛋白名称：
• Phospholipase D1

• Human Gene Id：
• 5337

• Human Swiss Prot No：
• Q13393

• Mouse Swiss Prot No：
• Q9Z280

• Rat Gene Id：
• 25096

• Rat Swiss Prot No：
• P70496

• 免疫原：
• Synthesized phospho-peptide around the phosphorylation site of human PC-PLD1 (phospho Thr147)

• 特异性：
• Phospho-PC-PLD1 (T147) Polyclonal Antibody detects endogenous levels of PC-PLD1 protein only when phosphorylated at T147.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• PLD1;Phospholipase D1;PLD 1;hPLD1;Choline phosphatase 1;Phosphatidylcholine-hydrolyzing phospholipase D1

• 实测条带：
• 120kD

• 背景：
• This gene encodes a phosphatidylcholine-specific phospholipase which catalyzes the hydrolysis of phosphatidylcholine in order to yield phosphatidic acid and choline. The enzyme may play a role in signal transduction and subcellular trafficking. Alternative splicing results in multiple transcript variants with both catalytic and regulatory properties. [provided by RefSeq, Sep 2011],

• 功能：
• catalytic activity:A phosphatidylcholine + H(2)O = choline + a phosphatidate.,enzyme regulation:Stimulated by phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate, activated by the phosphokinase C-alpha, by the ADP-ribosylation factor-1 (ARF-1), and to a lesser extent by GTP-binding proteins: RHO A, RAC-1 and CDC42. Inhibited by oleate.,function:Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic.,online information:Phospholipase D entry,similarity:Belongs to the phospholipase D family.,similarity:Contains 1 PH domain.,similarity:Contains 1 PX (phox homology) domain.,similarity:Contains 2 PLD phosphodiesterase domains.,subunit:Interacts with PIP5K1A.,tissue specificity:Expressed abundant

• 细胞定位：
• Cytoplasm, perinuclear region . Endoplasmic reticulum membrane ; Lipid-anchor ; Cytoplasmic side . Golgi apparatus membrane ; Lipid-anchor ; Cytoplasmic side . Late endosome membrane ; Lipid-anchor ; Cytoplasmic side .

• 组织表达：
• Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine.

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