RPA p32 (phospho Ser33) Polyclonal Antibody
- 货号：YP0476
- 应用：WB;IHC
- 种属：Human;Mouse;Rat
- 简介：
- >>DNA replication;>>Nucleotide excision repair;>>Mismatch repair;>>Homologous recombination;>>Fanconi anemia pathway
- 蛋白名称：
- Replication protein A 32 kDa subunit
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human RFA2 around the phosphorylation site of Ser33. AA range:1-50
- 特异性：
- Phospho-RPA p32 (S33) Polyclonal Antibody detects endogenous levels of RPA p32 protein only when phosphorylated at S33.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500-2000;IHC 1:50-300
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- RPA2;REPA2;RPA32;RPA34;Replication protein A 32 kDa subunit;RP-A p32;Replication factor A protein 2;RF-A protein 2;Replication protein A 34 kDa subunit;RP-A p34
- 背景：
- function:Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.,PTM:Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2.,subcellular location:Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.,subunit:Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN.,
- 功能：
- function:Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.,PTM:Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2.,subcellular location:Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.,subunit:Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN.,
- 细胞定位：
- Nucleus . Nucleus, PML body . Redistributes to discrete nuclear foci upon DNA damage in an ATR-dependent manner. .
- 组织表达：
- Kidney,Lung,Muscle,
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- Western Blot analysis of MCF-7 cells using Phospho-RPA p32 (S33) Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Western blot analysis of lysates from NIH/3T3 cells treated with Adriamycin 0.5ug/ml 24h, using RFA2 (Phospho-Ser33) Antibody. The lane on the right is blocked with the phospho peptide.
- Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
