Myf-5 (phospho Ser49) Polyclonal Antibody

    • 货号:YP0395
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse
      • 靶点:
      • Myf-5
      • 简介:
      • >>Signaling pathways regulating pluripotency of stem cells
      • 基因名称:
      • MYF5
      • 蛋白名称:
      • Myogenic factor 5
      • Human Gene Id:
      • 4617
      • Human Swiss Prot No:
      • P13349
      • Mouse Swiss Prot No:
      • P24699
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human Myogenic Factor 5 around the phosphorylation site of Ser49. AA range:21-70
      • 特异性:
      • Phospho-Myf-5 (S49) Polyclonal Antibody detects endogenous levels of Myf-5 protein only when phosphorylated at S49.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • MYF5;BHLHC2;Myogenic factor 5;Myf-5;Class C basic helix-loop-helix protein 2;bHLHc2
      • 实测条带:
      • 38kD
      • 背景:
      • function:Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein.,
      • 功能:
      • function:Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein.,
      • 细胞定位:
      • Nucleus .
      • 组织表达:
      • Skeletal muscle,
      • 产品图片
      • Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
      • Western blot analysis of lysates from HeLa cells, using Myogenic Factor 5 (Phospho-Ser49) Antibody. The lane on the right is blocked with the phospho peptide.