KIF20A (phospho Ser528) Polyclonal Antibody
- 货号:YP0373
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Rat;Mouse;
- 靶点:
- KIF20A
- 基因名称:
- KIF20A
- 蛋白名称:
- Kinesin-like protein KIF20A
- Human Gene Id:
- 10112
- Human Swiss Prot No:
- O95235
- Mouse Swiss Prot No:
- P97329
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human KIF20A around the phosphorylation site of Ser528. AA range:501-550
- 特异性:
- Phospho-KIF20A (S528) Polyclonal Antibody detects endogenous levels of KIF20A protein only when phosphorylated at S528.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 浓度:
- 1 mg/ml
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- KIF20A;MKLP2;RAB6KIFL;Kinesin-like protein KIF20A;GG10_2;Mitotic kinesin-like protein 2;MKlp2;Rab6-interacting kinesin-like protein;Rabkinesin-6
- 实测条带:
- 100kD
- 背景:
- function:Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.,similarity:Belongs to the kinesin-like protein family.,similarity:Contains 1 kinesin-motor domain.,
- 功能:
- function:Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.,similarity:Belongs to the kinesin-like protein family.,similarity:Contains 1 kinesin-motor domain.,
- 细胞定位:
- Golgi apparatus . Cytoplasm, cytoskeleton, spindle . Localizes to the spindle midzone during anaphase and telophase. .
- 组织表达:
- Brain,Endothelial cell,Epithelium,Uterus,
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- Antibody-FAQs
- 产品图片
- Western Blot analysis of 293 cells using Phospho-KIF20A (S528) Polyclonal Antibody
- Western blot analysis of lysates from 293 cells, using KIF20A (Phospho-Ser528) Antibody. The lane on the right is blocked with the phospho peptide.
- Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).