• 靶点：
• Sp1

• 简介：
• >>Endocrine resistance;>>Mitophagy - animal;>>TGF-beta signaling pathway;>>Estrogen signaling pathway;>>Cortisol synthesis and secretion;>>Parathyroid hormone synthesis, secretion and action;>>Cushing syndrome;>>Huntington disease;>>Spinocerebellar ataxia;>>Human cytomegalovirus infection;>>Pathways in cancer;>>Transcriptional misregulation in cancer;>>Breast cancer;>>Choline metabolism in cancer;>>Diabetic cardiomyopathy

• 基因名称：
• SP1

• 蛋白名称：
• Transcription factor Sp1

• Human Gene Id：
• 6667

• Human Swiss Prot No：
• P08047

• Mouse Gene Id：
• 20683

• Mouse Swiss Prot No：
• O89090

• Rat Gene Id：
• 24790

• Rat Swiss Prot No：
• Q01714

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human SP1 around the phosphorylation site of Thr453. AA range:421-470

• 特异性：
• Phospho-Sp1 (T453) Polyclonal Antibody detects endogenous levels of Sp1 protein only when phosphorylated at T453.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• SP1;TSFP1;Transcription factor Sp1

• 实测条带：
• 90kD

• 背景：
• The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014],

• 功能：
• function:Binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. Can interact with G/C-rich motifs from serotonin receptor promoter.,PTM:O-glycosylated; contains N-acetylglucosamine side chains.,similarity:Belongs to the Sp1 C2H2-type zinc-finger protein family.,similarity:Contains 3 C2H2-type zinc fingers.,subunit:Interacts with ATF7IP, ATF7IP2, POGZ, HCFC1, AATF and PHC2. Interacts with varicella-zoster virus IE62 protein and HIV-1 Vpr. Interacts with SV40 VP2/3 proteins. Interacts with SV40 major capsid protein VP1; this interaction leads to a cooperativity between the two proteins in DNA binding.,

• 细胞定位：
• Nucleus. Cytoplasm. Nuclear location is governed by glycosylated/phosphorylated states. Insulin promotes nuclear location, while glucagon favors cytoplasmic location.

• 组织表达：
• Up-regulated in adenocarcinomas of the stomach (at protein level). Isoform 3 is ubiquitously expressed at low levels.

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