• 靶点：
• CREB-1

• 简介：
• >>cGMP-PKG signaling pathway;>>cAMP signaling pathway;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Adrenergic signaling in cardiomyocytes;>>Osteoclast differentiation;>>Antigen processing and presentation;>>TNF signaling pathway;>>Circadian rhythm;>>Circadian entrainment;>>Thermogenesis;>>Cholinergic synapse;>>Dopaminergic synapse;>>Insulin secretion;>>Estrogen signaling pathway;>>Melanogenesis;>>Thyroid hormone synthesis;>>Glucagon signaling pathway;>>Renin secretion;>>Aldosterone synthesis and secretion;>>Relaxin signaling pathway;>>Cortisol synthesis and secretion;>>Parathyroid hormone synthesis, secretion and action;>>Insulin resistance;>>Cushing syndrome;>>Growth hormone synthesis, secretion and action;>>Vasopressin-regulated water reabsorption;>>Huntington disease;>>Prion disease;>>Cocaine addiction;>>Amphetamine addiction;>>Alcoholism;>>Tuberculosis;>>Hepatitis B;>>Human cytomegalovirus infection;>>Human papillomavirus infection;>>Human

• 基因名称：
• CREB1

• 蛋白名称：
• Cyclic AMP-responsive element-binding protein 1

• Human Gene Id：
• 1385

• Human Swiss Prot No：
• P16220

• Mouse Gene Id：
• 12912

• Mouse Swiss Prot No：
• Q01147

• Rat Gene Id：
• 81646

• Rat Swiss Prot No：
• P15337

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human CREB around the phosphorylation site of Ser133. AA range:100-149

• 特异性：
• Phospho-CREB-1 (S133) Polyclonal Antibody detects endogenous levels of CREB-1 protein only when phosphorylated at S133.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. Immunoprecipitation: 2-5 ug:mg lysate. ELISA: 1:10000. Not yet tested in other applications.

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• CREB1;Cyclic AMP-responsive element-binding protein 1;CREB-1;cAMP-responsive element-binding protein 1

• 实测条带：
• 37kD

• 背景：
• This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016],

• 功能：
• disease:A chromosomal aberration involving CREB1 is associated with angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.,function:This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transciption activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.,PTM:Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.,similarit

• 细胞定位：
• Nucleus .

• 组织表达：
• Eye,Placenta,Spleen,Testis,

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Cyclooxygenase-2 up-regulates hepatic somatostatin receptor 2 expression. Scientific Reports Sci Rep-Uk. 2018 Jul;8(1):1-11 WB Rat,Human cirrhotic liver L02 cell
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Fetal exposure to dichloroacetic acid and impaired cognitive function in the adulthood. Brain and Behavior Brain Behav. 2020 Oct;10(10):e01801 WB Rat 1 : 1000 Hippocampal tissue
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Dichloroacetic acid-induced dysfunction in rat hippocampus and the protective effect of curcumin. METABOLIC BRAIN DISEASE Metab Brain Dis. 2021 Apr;36(4):545-556 WB Rat 1:800 Hippocampus
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Developmental Exposure to Atrazine Impairs Spatial Memory and Downregulates the Hippocampal D1 Dopamine Receptor and cAMP-Dependent Signaling Pathway in Rats. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Int J Mol Sci. 2018 Aug;19(8):2241 WB Rat 1:1000 hippocampal tissue
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miR-709 inhibits GHRP6 induced GH synthesis by targeting PRKCA in pituitary. MOLECULAR AND CELLULAR ENDOCRINOLOGY Mol Cell Endocrinol. 2020 Apr;506:110763 WB Rat GH3 cell
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Tanshinone IIA Improves Depression-like Behavior in Mice by Activating the ERK-CREB-BDNF Signaling Pathway. NEUROSCIENCE Neuroscience. 2020 Mar;430:1 WB Mouse brain
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