CaMKIV (phospho Thr200) Polyclonal Antibody

    • 货号:YP0043
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse;Rat
      • 靶点:
      • CaMKIV
      • 简介:
      • >>Calcium signaling pathway;>>cAMP signaling pathway;>>Longevity regulating pathway;>>Apelin signaling pathway;>>Osteoclast differentiation;>>Long-term potentiation;>>Neurotrophin signaling pathway;>>Cholinergic synapse;>>Oxytocin signaling pathway;>>Aldosterone synthesis and secretion;>>Amphetamine addiction;>>Alcoholism;>>Glioma
      • 基因名称:
      • CAMK4
      • 蛋白名称:
      • Calcium/calmodulin-dependent protein kinase type IV
      • Human Gene Id:
      • 814
      • Human Swiss Prot No:
      • Q16566
      • Mouse Swiss Prot No:
      • P08414
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human CaMK4 around the phosphorylation site of Thr196/200. AA range:166-215
      • 特异性:
      • Phospho-CaMKIV (T200) Polyclonal Antibody detects endogenous levels of CaMKIV protein only when phosphorylated at T200.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • CAMK4;CAMK;CAMK-GR;CAMKIV;Calcium/calmodulin-dependent protein kinase type IV;CaMK IV;CaM kinase-GR
      • 实测条带:
      • 60kD
      • 背景:
      • The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. [provided by RefSeq, Jul 2008],
      • 功能:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activated by Ca(2+)/calmodulin. Binding of calmodulin may releave intrasteric autoinhibition. Must be phosphorylated to be maximally active. Phosphorylated by CAMKK1 or CAMKK2. Autophosphorylation of the N-terminus is required for full activation. In part, activity is independent on Ca(2+)/calmodulin and autophosphorylation of Ser-336 allows to switch to a Ca(2+)/calmodulin-independent state (By similarity). Probably inactivated by serine/threonine protein phosphatase 2A.,function:Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role i
      • 细胞定位:
      • Cytoplasm. Nucleus. Localized in hippocampal neuron nuclei. In spermatids, associated with chromatin and nuclear matrix (By similarity). .
      • 组织表达:
      • Expressed in brain, thymus, CD4 T-cells, testis and epithelial ovarian cancer tissue.

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      • 产品图片
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CaMK4 (Phospho-Thr196/200) Antibody
      • Immunofluorescence analysis of HeLa cells, using CaMK4 (Phospho-Thr196/200) Antibody. The picture on the right is blocked with the phospho peptide.
      • Immunohistochemistry analysis of paraffin-embedded human brain, using CaMK4 (Phospho-Thr196/200) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from K562 cells treated with H2O2 100uM 30', using CaMK4 (Phospho-Thr196/200) Antibody. The lane on the right is blocked with the phospho peptide.