• 靶点：
• CDK1/CDC2

• 简介：
• >>Cell cycle;>>Oocyte meiosis;>>p53 signaling pathway;>>Cellular senescence;>>Gap junction;>>Progesterone-mediated oocyte maturation;>>Human immunodeficiency virus 1 infection;>>Viral carcinogenesis

• 基因名称：
• CDK1

• 蛋白名称：
• Cyclin-dependent kinase 1

• Human Gene Id：
• 983

• Human Swiss Prot No：
• P06493

• Mouse Gene Id：
• 12534

• Mouse Swiss Prot No：
• P11440

• Rat Gene Id：
• 54237

• Rat Swiss Prot No：
• P39951

• 特异性：
• endogenous

• 组成：
• PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

• 来源：
• Monoclonal, rabbit, IgG, Kappa

• 稀释：
• IHC 1:1000-1:4000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200;

• 纯化工艺：
• Protein A

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• CDK1;CDC2;CDC28A;CDKN1;P34CDC2;Cyclin-dependent kinase 1;CDK1;Cell division control protein 2 homolog;Cell division protein kinase 1;p34 protein kinase

• 分子量：
• 34kD

• 实测条带：
• 34kD

• 背景：
• cyclin dependent kinase 1(CDK1) Homo sapiens The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009],

• 功能：
• catalytic activity:ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.,catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-161 activates it.,function:Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with DLGAP5. Isoform 2 is unable to complex with c

• 细胞定位：
• Cytoplasm, Nucleus

• 组织表达：
• Isoform 2 is found in breast cancer tissues.

Phosphoproteome Profiling Revealed the Importance of mTOR Inhibition on CDK1 Activation to Further Regulate Cell Cycle Progression. JOURNAL OF PROTEOME RESEARCH J Proteome Res. 2021;20(5):2329–2339 WB，IP Human 1:500 HeLa cell
货号：YM8251

RhoB Acts as a Tumor Suppressor That Inhibits Malignancy of Clear Cell Renal Cell Carcinoma. PLoS One Plos One. 2016 Jul;11(7):e0157599 WB Human 1:1000 A498 cell, Caki-2 cell, 786-O cell, 769-P cell, Caki-1 cell
货号：YM8251

5,6-Dihydroxy-3,7,4′-trimethoxyflavonol induces G2/M cell cycle arrest and apoptosis in human hepatocellular carcinoma cells. JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH J Asian Nat Prod Res. 2016;18(11):1079-1090 WB Human 1:1000 HepG-2 cell
货号：YM8251

5, 6-Dihydroxy-3, 7, 4′-trimethoxyflavonol induces G2/M cell cycle arrest and apoptosis in human hepatocellular carcinoma cells." Journal of Asian natural products research 18.11 (2016): 1079-1090.
货号：YM8251

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