PI3-Kinase p85 α (PT0082R) rabbit mAb
- 货号:YM8045
- 应用:WB;IHC;IF;IP;ELISA
- 种属:Human; Mouse; Rat;
- 靶点:
- PI3 Kinase P85α
- 简介:
- >>EGFR tyrosine kinase inhibitor resistance;>>Endocrine resistance;>>Platinum drug resistance;>>ErbB signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>cAMP signaling pathway;>>Chemokine signaling pathway;>>HIF-1 signaling pathway;>>FoxO signaling pathway;>>Phosphatidylinositol signaling system;>>Sphingolipid signaling pathway;>>Phospholipase D signaling pathway;>>Autophagy - animal;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Apoptosis;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Cellular senescence;>>Axon guidance;>>VEGF signaling pathway;>>Osteoclast differentiation;>>Focal adhesion;>>Signaling pathways regulating pluripotency of stem cells;>>Platelet activation;>>Neutrophil extracellular trap formation;>>Toll-like receptor signaling pathway;>>C-type lectin receptor signaling pathway;>>JAK-STAT signaling pathway;>>Natural killer cell mediated cytotoxicity;>>T cell receptor signaling pathway;>
- 基因名称:
- PIK3R1
- 蛋白名称:
- Phosphatidylinositol 3-kinase regulatory subunit alpha (PI3-kinase regulatory subunit alpha) (PI3K regulatory subunit alpha) (PtdIns-3-kinase regulatory subunit alpha) (Phosphatidylinositol 3-kinase 8
- Human Gene Id:
- 5295
- Human Swiss Prot No:
- P27986
- Mouse Swiss Prot No:
- P26450
- Rat Swiss Prot No:
- Q63787
- 特异性:
- endogenous
- 组成:
- PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
- 来源:
- Monoclonal, rabbit, IgG, Kappa
- 稀释:
- IHC 1:200-1000,WB 1:1000-5000,IF 1:200-1000,ELISA 1:5000-20000,IP 1:50-200
- 纯化工艺:
- Protein A
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- PIK3R1;GRB1;Phosphatidylinositol 3-kinase regulatory subunit alpha;PI3-kinase regulatory subunit alpha;PI3K regulatory subunit alpha;PtdIns-3-kinase regulatory subunit alpha;Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha;PI3-kinase subunit p85-alpha;PtdIns-3-kinase regulatory subunit p85-alpha
- 分子量:
- 84kD
- 实测条带:
- 84kD
- 背景:
- Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011],
- 功能:
- disease:Defects in PIK3R1 are a cause of severe insulin resistance.,domain:The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.,function:Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.,PTM:Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.,similarity:Belongs to the PI3K p85 subunit family.,similarity:Contains 1 Rho-GAP domain.,similarity:Contains 1 SH3 domain.,similarity:Contains 2 SH2 domains.,subunit:Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunits. Interacts with phosphorylated TOM1L1. Interacts with phosphorylat
- 细胞定位:
- nucleus,cytoplasm,cis-Golgi network,cytosol,plasma membrane,cell-cell junction,phosphatidylinositol 3-kinase complex,phosphatidylinositol 3-kinase complex, class IA,membrane,perinuclear endoplasmic reticulum membrane,
- 组织表达:
- Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).
Proteomic Study on the Reproductive Toxicity of Tripterygium Glycosides in Rats WB Rat testis tissue /
货号:YM8045
Melatonin treatment improves human umbilical cord mesenchymal stem cell therapy in a mouse model of type II diabetes mellitus via the PI3K/AKT signaling pathway. Stem Cell Research & Therapy2022 Dec;13(1):1-15. Human,Mouse 1:1200 liver tissue hUC-MSC
货号:YM8045
Irisin Suppresses Nicotine-Mediated Atherosclerosis by Attenuating Endothelial Cell Migration, Proliferation, Cell Cycle Arrest, and Cell Senescence. Frontiers in Cardiovascular Medicine2022; 9: 851603. Human 1:4000 HUVECs
货号:YM8045
Effects of D-Chiro-Inositol on Glucose Metabolism in db/db Mice and the Associated Underlying Mechanisms. Frontiers in Pharmacology Front Pharmacol. 2020 Mar;11:354 IHC Mouse 1:200 liver tissues
货号:YM8045
Induction of PI3K/Akt-Mediated Apoptosis in Osteoclasts Is a Key Approach for Buxue Tongluo Pills to Treat Osteonecrosis of the Femoral Head.. Frontiers in Pharmacology Front Pharmacol. 2021 Nov;12:729909-729909 WB Mouse 1:500,1:1000 RAW 264.7 cell
货号:YM8045
iTRAQ-Based Proteomics Reveals Gu-Ben-Fang-Xiao Decoction Alleviates Airway Remodeling via Reducing Extracellular Matrix Deposition in a Murine Model of Chronic Remission Asthma. Frontiers in Pharmacology Front Pharmacol. 2021; 12: 588588 WB Mouse 1:1000 Lung
货号:YM8045
5‐FU inhibits migration and invasion of CRC cells through PI3K/AKT pathway regulated by MARCH1. CELL BIOLOGY INTERNATIONAL Cell Biol Int. 2021 Feb;45(2):368-381 WB Human Colorectal cancer(CRC ) tissues sw480s,DLD-1s,NCM460s
货号:YM8045
An Integrated Approach Based on Network Pharmacology Combined with Experimental Verification Reveals AMPK/PI3K/Akt Signaling is an Important Way for the Anti-Type 2 Diabetic Activity of Silkworm Excrement. Diabetes Metabolic Syndrome and Obesity-Targets and Therapy Diabet Metab Synd Ob. 2021 Feb;14:601-616 WB Human 1:1000 HepG2 cell
货号:YM8045
Scutellarin Prevents Nonalcoholic Fatty Liver Disease (NAFLD) and Hyperlipidemia via PI3K/AKT-Dependent Activation of Nuclear Factor (Erythroid-Derived 2)-Like 2 (Nrf2) in Rats. MEDICAL SCIENCE MONITOR Med Sci Monitor. 2017; 23: 5599–5612 WB Rat 1: 400 liver tissue
货号:YM8045
Effects of Agriophyllum squarrosum extracts on glucose metabolism in KKAy mice and the associated underlying mechanisms. JOURNAL OF ETHNOPHARMACOLOGY J Ethnopharmacol. 2019 Sep;241:112009 WB Mouse 1:1000 liver
货号:YM8045
Long Non-coding RNA GAS5 Maintains Insulin Secretion by Regulating Multiple miRNAs in INS-1 832/13 Cells. Frontiers in Molecular Biosciences Front Mol Biosci. 2020; 7: 559267 WB Rat 1 : 1000 INS-1 832/13 cell
货号:YM8045
Irisin ameliorates nicotine-mediated atherosclerosis via inhibition of the PI3K pathway. Annals of Translational Medicine Ann Transl Med. 2021 May; 9(9): 805 WB Mouse 1:10000 Aortic
货号:YM8045
The role and regulatory mechanism of autophagy in hippocampal nerve cells of piglet damaged by deoxynivalenol. TOXICOLOGY IN VITRO Toxicol In Vitro. 2020 Aug;66:104837 WB Pig 1:1000 piglet hippocampal nerve cells (PHNCs)
货号:YM8045
Sattar, Hamid, et al. "Selenoprotein-U (SelU) knockdown triggers autophagy through PI3K–Akt–mTOR pathway inhibition in rooster Sertoli cells." Metallomics 10.7 (2018): 929-940.
货号:YM8045
Yang, Jie, et al. "Selenium deficiency-induced thioredoxin suppression and thioredoxin knock down disbalanced insulin responsiveness in chicken cardiomyocytes through PI3K/Akt pathway inhibition." Cellular signalling 38 (2017): 192-200.
货号:YM8045
Wang, Wei-wei, et al. "Telmisartan reduces atrial arrhythmia susceptibility through the regulation of RAS–ERK and PI3K–Akt–eNOS pathways in spontaneously hypertensive rats." Canadian journal of physiology and pharmacology 93.8 (2015): 657-665.
货号:YM8045
Irisin ameliorates nicotine-mediated atherosclerosis via inhibition of the PI3K pathway. Annals of Translational Medicine Ann Transl Med. 2021 May; 9(9): 805 WB Mouse 1:10000 Aortic
货号:YM8045
5‐FU inhibits migration and invasion of CRC cells through PI3K/AKT pathway regulated by MARCH1. CELL BIOLOGY INTERNATIONAL Cell Biol Int. 2021 Feb;45(2):368-381 WB Human Colorectal cancer(CRC ) tissues sw480s,DLD-1s,NCM460s
货号:YM8045
5‐FU inhibits migration and invasion of CRC cells through PI3K/AKT pathway regulated by MARCH1. CELL BIOLOGY INTERNATIONAL Cell Biol Int. 2021 Feb;45(2):368-381 WB Human Colorectal cancer(CRC ) tissues sw480s,DLD-1s,NCM460s
货号:YM8045
Irisin ameliorates nicotine-mediated atherosclerosis via inhibition of the PI3K pathway. Annals of Translational Medicine Ann Transl Med. 2021 May; 9(9): 805 WB Mouse 1:10000 Aortic
货号:YM8045
Arsenic Trioxide-loaded nanoparticles Enhance the Chemosensitivity of Gemcitabine in Pancreatic Cancer via Reversal of Pancreatic Stellate Cells Desmoplasia through Targeting AP4/Galectin-1 Pathway Biomaterials Science Kaihong Huang WB Human
货号:YM8045
Amelioration of Lung Fibrosis by Total Flavonoids of Astragalus via Inflammatory Modulation and Epithelium Regeneration AMERICAN JOURNAL OF CHINESE MEDICINE Min Li WB Human,Mouse lung tissues A549 cells
货号:YM8045
Crosstalk between autophagy inhibitor and salidroside-induced apoptosis: A novel strategy for autophagy-based treatment of hepatocellular cancer. INTERNATIONAL IMMUNOPHARMACOLOGY Haixiang Su WB Human 1:1000 HepG2 cell,97H cell
货号:YM8045
Multi-omics revealed the mechanisms of Codonopsis pilosula aqueous extract in improving UC through blocking abnormal activation of PI3K/Akt signaling pathway. JOURNAL OF ETHNOPHARMACOLOGY Fude Yang IHC Rat intestinal mucosa
货号:YM8045
Cucurbitacin E reduces IL-1β-induced inflammation and cartilage degeneration by inhibiting the PI3K/Akt pathway in osteoarthritic chondrocytes Journal of Translational Medicine Wang Lin WB Human 1:1000 Chondrocytes
货号:YM8045
MiR-21-5p ameliorates Busulfan-induced testicular dysfunction and maintains spermatogenesis1 Journal of Integrative Agriculture Meng-fei ZHANG WB Goat Male germ stem cells (mGSCs)
货号:YM8045
Synthesis of peiminine derivatives and its effect on A549 lung cancer-bearing mice Chemical Biology & Drug Design Xin Jin WB Mouse A549 cell-Xenograft
货号:YM8045
Nasopharyngeal carcinoma with non-squamous phenotype may be a variant of nasopharyngeal squamous cell carcinoma after inhibition of EGFR/PI3K/AKT/mTOR pathway HISTOLOGY AND HISTOPATHOLOGY Jiahe Wang IHC Human non-squamous immuno-phenotype nasopharyngeal carcinoma (NSNPC) tissue
货号:YM8045
Network Pharmacology Analysis, Molecular Docking Integrated Experimental Verification Reveal the Mechanism of Gynostemma pentaphyllum in the Treatment of Type II Diabetes by Regulating the IRS1/PI3K/Akt Signaling Pathway CURRENT ISSUES IN MOLECULAR BIOLOGY Songqin Yang WB Human HepG2 cell
货号:YM8045
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- May 20-2022
- Cell-Based ELISA│解您多样本WB检测之困扰
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- Antibody-FAQs
- 产品图片
- Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PI3-Kinase p85 α (PT0082R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: HepG2 Lane 3: Mouse brain Lane 4: Rat brain Predicted band size: 84kDa Observed band size: 84kDa
- Mouse testis was stained with Anti-PI3-Kinase p85 α (PT0082R) rabbit Antibody
- Human placenta was stained with Anti-PI3-Kinase p85 α (PT0082R) rabbit Antibody
- Fan, Hua, et al. "Scutellarin Prevents Nonalcoholic Fatty Liver Disease (NAFLD) and Hyperlipidemia via PI3K/AKT-Dependent Activation of Nuclear Factor (Erythroid-Derived 2)-Like 2 (Nrf2) in Rats." Medical science monitor: international medical journal of experimental and clinical research 23 (2017): 5599.