- 靶点：
- Ki-67
- 基因名称：
- MKI67
- 蛋白名称：
- Ki 67
- Human Gene Id：
- 4288
- Human Swiss Prot No：
- P46013
- 免疫原：
- Synthesized peptide derived from human Ki-67 AA range:1200-1400
- 特异性：
- This antibody detects endogenous levels of Ki-67
- 组成：
- PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
- 来源：
- Monoclonal, Rabbit IgG1, Kappa
- 稀释：
- IHC 1:100-500, WB 1:500-1000, ELISA 1:5000-20000
- 纯化工艺：
- Recombinant Expression and Affinity purified
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- Antigen identified by monoclonal Ki 67;Antigen identified by monoclonal Ki-67;Antigen KI-67;Antigen KI67;Antigen Ki67;KI67_HUMAN;KIA;Marker of prolifeRation Ki-67;MIB 1;MIB;MKI67;PPP1R105;ProlifeRation marker protein Ki-67;ProlifeRation related Ki 67 antigen;Protein phosphatase 1 regulatory subunit 105;RP11-380J17.2
- 分子量：
- 359kD
- 背景：
- This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009],
- 功能：
- developmental stage:Expression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected.,function:Thought to be required for maintaining cell proliferation.,online information:Ki-67 entry,similarity:Contains 1 FHA domain.,subcellular location:Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.,subunit:Interacts with KIF15. Binds through the FHA domain to MKI67IP.,
- 细胞定位：
- Nuclear
- 组织表达：
- Nuclear
Predictive Roles of ADAM17 in Patient Survival and Immune Cell Infiltration in Hepatocellular Carcinoma INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Tianlong Ding IHC Mouse 1:150 MHCC97-H cell-Xenograft
货号：YM7002
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- Antibody-FAQs
- 产品图片
- Fluorescence multiplex immunohistochemical analysis of Human tonsil tissue (formalin-fixed paraffin-embedded section). The immunostaining was performed by Pentuple-Fluorescence kit (RS0038, Immunoway). CK-pan mouse mAb(YM6815 Immunoway) green, Ki-67 rabbit mAb(YM7002 Immunoway) red, Iba 1 mouse mAb(YM4765 Immunoway) purple,CD8 a mouse mAb(YM4815 Immunoway) cyan, CD20 mouse mAb(YM4814 Immunoway) yellow, The section was incubated in 5 rounds of staining; sequentially for Anti-antibodies; each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Immunoway YS0004, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (Excilone).
