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HP-1γ mouse Monoclonal Antibody(3B9)

货号：YM3742
应用：WB;IHC;IF
种属：Human;Mouse;Rat

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靶点：
HP1γ

简介：
>>Shigellosis

基因名称：
CBX3

蛋白名称：
Chromobox protein homolog 3 (HECH) (Heterochromatin protein 1 homolog gamma) (HP1 gamma) (Modifier 2 protein)

Human Gene Id：
11335

Human Swiss Prot No：
Q13185

Mouse Swiss Prot No：
P23198

免疫原：
Recombinant Protein of HP-1γ

特异性：
The antibody detects endogenous HP-1γ protein

组成：
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

来源：
Monoclonal, Mouse

稀释：
WB 1:500-2000,IHC 1:50-300. IF 1:50-200

纯化工艺：
The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen.

浓度：
1 mg/ml

储存：
-15°C to -25°C/1 year(Do not lower than -25°C)

其他名称：
Chromobox protein homolog 3 (HECH;Heterochromatin protein 1 homolog gamma;HP1 gamma;Modifier 2 protein)

实测条带：
24kD

背景：
At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.[provided by RefSeq, Mar 2011],

功能：
function:Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.,PTM:Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.,similarity:Contains 2 chromo domains.,subcellular location:Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis.,subunit:Binds directly to CHAF1A. Interacts with histone H3 methylated at 'Lys-9'. Part of the E2F6.com-1 complex in G0 phase composed of E2F

细胞定位：
Nucleus . Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis (Potential). .

组织表达：
Bone marrow,Brain,Cajal-Retzius cell,Epithelium,Liver,Placenta,

Comprehensive Analysis of the Expression and Prognosis Value of Chromobox Family Members in Clear Cell Renal Cell Carcinoma. Frontiers in Oncology Front Oncol. 2021 Jul;0:2978 WB，IHC Human 1:200，1:1000 786-O cell， 769-P cell,HK2 cell
货号：YM3742