• 靶点：
• PARP

• 简介：
• >>Base excision repair;>>NF-kappa B signaling pathway;>>Apoptosis;>>Necroptosis;>>Diabetic cardiomyopathy

• 基因名称：
• PARP1

• 蛋白名称：
• Poly [ADP-ribose] polymerase 1

• Human Gene Id：
• 142

• Human Swiss Prot No：
• P09874

• Mouse Swiss Prot No：
• P11103

• Rat Gene Id：
• 25591

• Rat Swiss Prot No：
• P27008

• 免疫原：
• Synthetic Peptide of Cleaved PARP

• 特异性：
• The antibody detects endogenous pro and active PARP protein.

• 组成：
• PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

• 来源：
• Monoclonal, Mouse

• 稀释：
• IF 1:50-200 WB 1:2000-5000 IHC 1:50-300

• 纯化工艺：
• The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• PARP1;ADPRT;PPOL;Poly [ADP-ribose] polymerase 1;PARP-1;ADP-ribosyltransferase diphtheria toxin-like 1;ARTD1;NAD(+) ADP-ribosyltransferase 1;ADPRT 1;Poly[ADP-ribose] synthase 1

• 实测条带：
• 116,89kD

• 背景：
• This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008],

• 功能：
• catalytic activity:NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.,function:Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.,miscellaneous:The ADP-D-ribosyl group of NAD(+) is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units.,PTM:Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Poly-ADP-ribosylated by PARP2.,similarity:Contains 1 BRCT

• 细胞定位：
• Nucleus . Nucleus, nucleolus . Chromosome . Localizes to sites of DNA damage. .

• 组织表达：
• Brain,Colon carcinoma,Fibroblast,Lung,Ovarian carcinoma,Skin,

Involvement of dysregulated Wip1 in manganese-induced p53 signaling and neuronal apoptosis. TOXICOLOGY LETTERS 2015 Mar 16 WB Rat 1:1000 brain
货号：YM3145

Pivotal roles of p53 transcription-dependent and -independent pathways in manganese-induced mitochondrial dysfunction and neuronal apoptosis. TOXICOLOGY AND APPLIED PHARMACOLOGY Toxicol Appl Pharm. 2014 Dec;281:294 WB Rat 1:2000 striatum
货号：YM3145

Sodium butyrate induces autophagic apoptosis of nasopharyngeal carcinoma cells by inhibiting AKT/mTOR signaling. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Biochem Bioph Res Co. 2019 Jun;514:64 WB Human 5–8F cell, HK-1 cell
货号：YM3145

DLC-1 induces mitochondrial apoptosis and epithelial mesenchymal transition arrest in nasopharyngeal carcinoma by targeting EGFR/Akt/NF-κB pathway. MEDICAL ONCOLOGY Med Oncol. 2015 Apr;32(4):1-7 WB Human 5-8F-DLC-1 cell
货号：YM3145

Marine bromophenol bis(2,3-dibromo-4,5-dihydroxybenzyl) ether, represses angiogenesis in HUVEC cells and in zebrafish embryos via inhibiting the VEGF signal systems. BIOMEDICINE & PHARMACOTHERAPY 2015 Sep 07 WB Human HUVECs
货号：YM3145

Sodium butyrate induces autophagic apoptosis of nasopharyngeal carcinoma cells by inhibiting AKT/mTOR signaling. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Biochem Bioph Res Co. 2019 Jun;514:64 WB Human 5–8F cell, HK-1 cell
货号：YM3145

Dietary antioxidant quercetin overcomes the acquired resistance of Sorafenib in Sorafenib-resistant hepatocellular carcinoma cells through epidermal growth factor receptor signaling inactivation. Zhou Fuqiong WB Mouse，Human 1:1000 Huh7 cell-xenograft Huh7 cell
货号：YM3145

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