• 靶点：
• PARP

• 简介：
• >>Base excision repair;>>NF-kappa B signaling pathway;>>Apoptosis;>>Necroptosis;>>Diabetic cardiomyopathy

• 基因名称：
• PARP1

• 蛋白名称：
• Poly [ADP-ribose] polymerase 1

• Human Gene Id：
• 142

• Human Swiss Prot No：
• P09874

• Mouse Swiss Prot No：
• P11103

• Rat Gene Id：
• 25591

• Rat Swiss Prot No：
• P27008

• 免疫原：
• Synthetic Peptide of PARP

• 特异性：
• The antibody detects endogenous PARP protein.

• 组成：
• PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

• 来源：
• Monoclonal, Mouse

• 稀释：
• WB 1:1000-3000

• 纯化工艺：
• The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• Poly [ADP-ribose] polymerase 1 (PARP-1) (EC 2.4.2.30) (ADP-ribosyltransferase diphtheria toxin-like 1) (ARTD1) (NAD(+) ADP-ribosyltransferase 1) (ADPRT 1) (Poly[ADP-ribose] synthase 1)

• 实测条带：
• 116kD

• 背景：
• This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008],

• 功能：
• catalytic activity:NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.,function:Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.,miscellaneous:The ADP-D-ribosyl group of NAD(+) is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units.,PTM:Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Poly-ADP-ribosylated by PARP2.,similarity:Contains 1 BRCT

• 细胞定位：
• Nucleus . Nucleus, nucleolus . Chromosome . Localizes to sites of DNA damage. .

• 组织表达：
• Brain,Colon carcinoma,Fibroblast,Lung,Ovarian carcinoma,Skin,

Modulations of Keap1-Nrf2 signaling axis by TIIA ameliorated the oxidative stress-induced myocardial apoptosis. FREE RADICAL BIOLOGY AND MEDICINE Free Radical Bio Med. 2018 Feb;115:191 WB Mouse Neonatal rat ventricular myocytes (NRVMs)
货号：YM3131

The induction of apoptosis and autophagy in human hepatoma SMMC-7721 cells by combined treatment with vitamin C and polysaccharides extracted from Grifola frondosa. APOPTOSIS 2017 Sep 11 WB Human SMMC-7721 cell
货号：YM3131

Coptisine Induces Apoptosis in Human Hepatoma Cells Through Activating 67-kDa Laminin Receptor/cGMP Signaling. Frontiers in Pharmacology 2018 May 18 WB Human 1:500 SMMC7721 cell, HepG2 Cell
货号：YM3131

High CHMP4B expression is associated with accelerated cell proliferation and resistance to doxorubicin in hepatocellular carcinoma. TUMOR BIOLOGY Tumor Biol. 2015 Apr;36(4):2569-2581 WB Human HepG2 cell, Huh7 HCC cell
货号：YM3131

High expression of PINK1 promotes proliferation and chemoresistance of NSCLC. ONCOLOGY REPORTS Oncol Rep. 2017 Apr;37(4):2137-2146 WB Human NSCLC tissues A549 cell, H1299 cell
货号：YM3131

Effect of metformin on apoptosis, cell cycle arrest migration and invasion of A498 cells. Molecular Medicine Reports 2014 Mar 31 WB Human A498 cell
货号：YM3131

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