- 靶点:
- Strep-Tag II
- 免疫原:
- Synthetic Peptide of Strep-Tag II
- 特异性:
- The antibody detects Strep-Tag II fusion proteins.
- 组成:
- PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
- 来源:
- Monoclonal, Mouse
- 稀释:
- WB 1:5000-10000 IP 1:50-200
- 纯化工艺:
- Recombinant Expression and Affinity purified
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- Strep-Tag II;Strep Tag II;
Urease of Aspergillus fumigatus Is Required for Survival in Macrophages and Virulence Microbiology Spectrum Daniel H. Scharf WB Escherichia coli
货号:YM3016
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- May 20-2022
- Cell-Based ELISA│解您多样本WB检测之困扰
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- Antibody-FAQs
- 产品图片
- Whole extracts were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Strep-Tag Ⅱ(7A10)antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: Rosetta transfected with an empty expression vector Lane 2: Rosetta transfected with a Strep-TagⅡ-tagged MBP protein Predicted band size: 55kDa Observed band size: 55kDa
- Strep-Tag Ⅱ was immunoprecipitated from Strep-Tag Ⅱ-MBP(Rosetta) lysate with anti-Strep-Tag Ⅱ antibody. Western blot was performed on the immunoprecipitate using anti-Strep-Tag Ⅱ antibody, and followed by the HRP-conjugated Goat anti-Mouse IgG Light chain antibody. Lane 1: Strep-Tag Ⅱ-MBP(Rosetta) lysate Lane 2: anti-Strep-Tag Ⅱ antibody IP in Strep-Tag Ⅱ-MBP(Rosetta) lysate Lane 3: Mouse monoclonal IgG (MNH209) in Strep-Tag Ⅱ-MBP(Rosetta) lysate.
- 1ug Strep II fusion protein+ Primary antibody dilution at 1) 1:5000 2) 1:10000