- 靶点：
- His-Tag
- 基因名称：
- His-Tag
- 蛋白名称：
- His Tag
- 免疫原：
- Synthetic peptide:HHHHHH conjugated to KLH.
- 特异性：
- The antibody detects C-terminal, internal, and N-terminal His-tag fusion proteins.
- 组成：
- PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
- 来源：
- Monoclonal, Mouse
- 稀释：
- WB 1:3000 IP: 1:200 IF 1:1000
- 纯化工艺：
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景：
- A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
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货号：YM3004
A PARylation-phosphorylation cascade promotes TOPBP1 loading and RPA-RAD51 exchange in homologous recombination CoIP Human /HeLa cell，U2OS cell
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Facile purification of active recombinant mouse cytosolic carboxypeptidase 6 from Escherichia coli WB Escherichia Coli 1:5000 /
货号：YM3004
Construction and evaluation of DNA vaccine encoding Ebola virus glycoprotein fused with lysosome-associated membrane protein. ANTIVIRAL RESEARCH Antivir Res. 2021 Sep;193:105141 WB Human 1:3000 HeLa cell
货号：YM3004
Novel Functional Recombinant Human Follicle-Stimulating Hormone Acquired from Goat Milk. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY J Agr Food Chem. 2021;69(9):2793–2804 Pull down，WB Goat 1:16000 Goat mammary glands GMECs
货号：YM3004
Cloning, identification of the two cytokine receptor family B subunits CRFB1 and CRFB5 from grass carp (Ctenopharyngodon idella). FISH & SHELLFISH IMMUNOLOGY Fish Shellfish Immun. 2015 Aug;45:211 WB Fish CIK cell
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Overexpression of neuregulin 1 in GABAergic interneurons results in reversible cortical disinhibition. Nature Communications Nat Commun. 2021 Jan;12(1):1-13 WB Mouse 1:2000 Prefrontal cotex
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HU, Jian-wen, et al. "Antibiotics and ELISA as a Hyphenated Technique for Screening High Production Cell Lines from Transfected HEK293 Cells for the Production of Recombinant Follicle Stimulating Hormone." 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017). Atlantis Press, 2017.
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AtHD2D, A Plant-specific Histone Deacetylase Involved in Abscisic Acid Response and Lateral Root Development JOURNAL OF EXPERIMENTAL BOTANY Zhaofen Han Pull down, WB Arabidopsis thaliana
货号：YM3004
Therapeutic potential of kaempferol on Streptococcus pneumoniae infection
货号：YM3004
CCP5 and CCP6 retain CP110 and negatively regulate ciliogenesis. Hui-Yuan Wu IP Human 1:2000 HEK293T cell
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The wheat VQ motif-containing protein TaVQ4-D positively regulates drought tolerance in transgenic plants. Dong-hong Min Pull down Wheat leaves
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WDR61 ablation triggers R-loop accumulation and suppresses breast cancer progression FEBS Journal Yayan Hou WB Escherichia coli
货号：YM3004
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- Antibody-FAQs
- 产品图片
- ADAR1 links R-loop homeostasis to ATR activation in replication stress response. NUCLEIC ACIDS RESEARCH Lei Shi WB,CoIP Human HeLa cell
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- Wang, YY., Zhao, B., Wu, MM. et al. Overexpression of neuregulin 1 in GABAergic interneurons results in reversible cortical disinhibition. Nat Commun 12, 278 (2021).
- 2ug His fusion protein+ Primary antibody dilution at 1) 1:5000 2) 1:10000
- IF analysis of 293 cells transfected with a His-tag protein,1:1000 dilution (blue DAPI, red anti-His)
- IP antibody use:5ug His Mouse IgG1 per ml Lysate,WB 1:3000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with His-tag fusion protein 3、IP (untransfected 293+anti-His mAb+ Protein G agarose) 4、IP (transfected 293+ normal Mouse IgG+Protein G agarose) 5、IP (transfected 293+anti-His mAb+ Protein G agarose) 6、Recombinant protein (E.coli)
