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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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- 免疫原:
- Purified recombinant human Phostensin protein fragments expressed in E.coli.
- 特异性:
- This antibody detects endogenous levels of Phostensin and does not cross-react with related proteins.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 纯化工艺:
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- HKMT1098;KIAA1949;Protein phosphatase 1 F actin cytoskeleton targeting subunit.
- 背景:
- Protein phosphatase-1 (PP1; see MIM 176875) interacts with regulatory subunits that target the enzyme to different cellular locations and change its activity toward specific substrates. Phostensin is a regulatory subunit that targets PP1 to F-actin (see MIM 102610) cytoskeleton (Kao et al., 2007 [PubMed 17374523]).[supplied by OMIM, Mar 2008],
- 功能:
- function:May target protein phosphatase 1 to F-actin cytoskeleton.,subunit:Interacts with Protein phosphatase 1 (PP1).,tissue specificity:Predominantly expressed in leukocytes and spleen.,
- 细胞定位:
- [Isoform 1]: Cytoplasm, cytoskeleton .; [Isoform 4]: Cytoplasm, cytoskeleton .
- 组织表达:
- Isoform 4 is predominantly expressed in leukocytes and spleen.
- Western blot detection of Phostensin in Hela,Raji,Ramos and Leucocyte cell lysates and using Phostensin mouse mAb (1:200 diluted).Predicted band size: 120KDa.Observed band size: 120KDa.