- 靶点：
- CHD3
- 基因名称：
- chd3
- Human Gene Id：
- 1107
- Human Swiss Prot No：
- Q12873
- 免疫原：
- Purified recombinant human CHD3 (C-terminus) protein fragments expressed in E.coli.
- 特异性：
- This antibody detects endogenous levels of CHD3 (C-terminus) and does not cross-react with related proteins.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Monoclonal, Mouse
- 稀释：
- wb 1:1000 icc 1:400
- 纯化工艺：
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
- 浓度：
- 1 mg/ml
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- 226;2600010P09Rik;AF020312;ATP dependent helicase CHD3;ATP-dependent helicase Chd3;CHD-3;Chd3;CHD3_HUMAN;Chd7;Chromodomain-helicase-DNA-binding protein 3;hZFH;MGC40857;Mi 2 autoantigen 240 kDa protein;Mi 2a;Mi-2 autoantigen 240 kDa protein;Mi2 ALPHA;Mi2-alpha;Prp7;Prp9 1;ZFH;Zinc finger helicase;zinc-finger helicase (Snf2-like).
- 实测条带：
- 260kD
- 背景：
- This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008],
- 功能：
- disease:One of the main antigens reacting with anti-MI-2 positive sera of dermatomyositis.,function:Probable transcription regulator.,sequence caution:Differs from position 1967 onward for unknown reasons.,similarity:Belongs to the SNF2/RAD54 helicase family.,similarity:Contains 1 helicase ATP-binding domain.,similarity:Contains 1 helicase C-terminal domain.,similarity:Contains 2 chromo domains.,similarity:Contains 2 PHD-type zinc fingers.,subunit:Central component of the nucleosome remodeling and histone deacetylase (NuRD) repressive complex. Interacts with TRIM28 and SERBP1. Interacts via its C-terminal region with HABP4.,tissue specificity:Widely expressed.,
- 细胞定位：
- Nucleus, PML body . Nucleus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Associates with centrosomes in interphase and mitosis. .
- 组织表达：
- Widely expressed.
A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis MOLECULAR CELL Kaiwen Bao IF Human HeLa cell
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- Antibody-FAQs
- 产品图片
- Western blot detection of CHD3 (C-terminus) in NTERA2,3T3,C6,K562 and Hela cell lysates using CHD3 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size: 260KDa.Observed band size: 260KDa.
- Immunocytochemistry staining of HeLa cells fixed with -20℃ Methanol and using CHD3 (C-terminus) mouse mAb (dilution 1:400).
- Immunoprecipitation analysis of Hela cell lysates using CHD3 mouse mAb.
