COX IV mouse mAb
- 货号：YM1222
- 应用：WB;FC;ICC;IP;IHC
- 种属：Human;Mouse;Rat;Hamster;Goat;Monkey
- 靶点：
- COX IV
- 简介：
- >>Oxidative phosphorylation;>>Metabolic pathways;>>Cardiac muscle contraction;>>Thermogenesis;>>Non-alcoholic fatty liver disease;>>Alzheimer disease;>>Parkinson disease;>>Amyotrophic lateral sclerosis;>>Huntington disease;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Chemical carcinogenesis - reactive oxygen species;>>Diabetic cardiomyopathy
- 基因名称：
- cox iv
- Human Gene Id：
- 1327
- Human Swiss Prot No：
- P13073
- Mouse Swiss Prot No：
- P19783
- 免疫原：
- A synthetic peptide corresponding to carboxyl terminal residues of human COX IV
- 特异性：
- This antibody detects endogenous levels of COX IV and does not cross-react with related proteins.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Monoclonal, Mouse
- 稀释：
- wb 1:1000 icc 1:150 fcm 1:100
- 纯化工艺：
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
- 浓度：
- 1 mg/ml
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- AL024441;COX 4;COX IV 1;COX IV;COX IV-1;Cox4;COX41_HUMAN;Cox4a;COX4B;COX4I1;COX4I2;COX4L2;COXIV;Cytochrome c oxidase polypeptide IV;Cytochrome c oxidase subunit 4 isoform 1 mitochondrial;Cytochrome c oxidase subunit 4 isoform 1, mitochondrial;Cytochrome C Oxidase subunit IV;Cytochrome c oxidase subunit IV isoform 1;Cytochrome c oxidase subunit IV isoform 2 (lung);Cytochrome c oxydase subunit;dJ857M17.2;MGC105470;MGC72016.
- 实测条带：
- 17kD
- 背景：
- Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes
- 功能：
- function:This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.,similarity:Belongs to the cytochrome c oxidase IV family.,tissue specificity:Ubiquitous.,
- 细胞定位：
- Mitochondrion inner membrane ; Single-pass membrane protein .
- 组织表达：
- Ubiquitous.
Chen, Zhida, et al. "Silencing Herg1 by shRNA inhibits the proliferation and invasion of osteosarcoma in vivo and in vitro." (2021).
货号：YM1222
- June 19-2018
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- June 19-2018
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- July 13-2018
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- July 13-2018
- Antibody-FAQs
- 产品图片
- Western blot detection of COX IV in Mouse skeletal muscel and Jurkat lysates using COX IV mouse mAb (1:1000 diluted). Predicted band size: 17KDa.Observed band size: 17KDa.
- Immunocytochemistry of HeLa cells using anti-COX IV mouse mAb diluted 1:150.
- Immunoprecipitation analysis of Hela cell lysates using COX IV mouse mAb.
- Immunohistochemical analysis of paraffin-embedded human colorectal carcinoma with COX IV Mouse mAb (4D11-B3-E8,1:50 diluted),showing cytoplasm localization.A high pressure mediated antigen retrieval step was performed in citrate buffer(pH6.0).
- Flow Cytometry analysis of K562 cells stained with COX4 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.
- Western blot detection of COX IV in Goat muscle,CHO-k1,COS7,3T3,Hela,C6 and K562 cell lysates using COX IV mouse mAb (1:5000 diluted).Predicted band size:17KDa.Observed band size:17KDa.
