THAP11 Monoclonal Antibody
- 货号：YM0617
- 应用：WB;IHC;IF;FCM;ELISA
- 种属：Human
- 蛋白名称：
- THAP domain-containing protein 11
- 免疫原：
- Purified recombinant fragment of human THAP11 expressed in E. Coli.
- 特异性：
- THAP11 Monoclonal Antibody detects endogenous levels of THAP11 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:500 - 1:2000. IHC 1:200 - 1:1000. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- THAP11;HRIHFB2206;THAP domain-containing protein 11
- 背景：
- The protein encoded by this gene contains a THAP domain, which is a conserved DNA-binding domain that has striking similarity to the site-specific DNA-binding domain (DBD) of Drosophila P element transposases. [provided by RefSeq, Jul 2008],
- 功能：
- function:Transcriptional repressor that plays a central role for embryogenesis and the pluripotency of embryonic stem (ES) cells. Sequence-specific DNA-binding factor that represses gene expression in pluripotent ES cells by directly binding to key genetic loci and recruiting epigenetic modifiers.,polymorphism:The length of the poly-Gln region is variable in the population.,similarity:Belongs to the THAP11 family.,similarity:Contains 1 THAP-type zinc finger.,subcellular location:May be regulated by shuttling of the protein between the cytoplasm and nucleus.,subunit:Interacts (via coiled coil domain) with HCFC1.,
- 细胞定位：
- Nucleus . Cytoplasm . May be regulated by shuttling of the protein between the cytoplasm and nucleus. .
- 组织表达：
- Brain,Neuroblastoma,Uterus,
- Western Blot analysis using THAP11 Monoclonal Antibody against HeLa (1) and NTERA-2 (2) cell lysate.
- Immunohistochemistry analysis of paraffin-embedded colon cancer tissues (left) and ovary cancer tissues (right) with DAB staining using THAP11 Monoclonal Antibody.
- Immunofluorescence analysis of NTERA-2 cells using THAP11 Monoclonal Antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Flow cytometric analysis of Hela cells using THAP11 Monoclonal Antibody (green) and negative control (purple).
