• 靶点：
• PDK1

• 简介：
• >>HIF-1 signaling pathway;>>Central carbon metabolism in cancer

• 基因名称：
• PDK1

• 蛋白名称：
• [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1 mitochondrial

• Human Gene Id：
• 5163

• Human Swiss Prot No：
• Q15118

• Mouse Swiss Prot No：
• Q8BFP9

• Rat Swiss Prot No：
• Q63065

• 免疫原：
• Purified recombinant fragment of human PDK1 expressed in E. Coli.

• 特异性：
• PDK1 Monoclonal Antibody detects endogenous levels of PDK1 protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Monoclonal, Mouse

• 稀释：
• WB 1:500 - 1:2000. IHC 1:200 - 1:1000. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.

• 纯化工艺：
• Affinity purification

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• PDK1;PDHK1;[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1; mitochondrial;Pyruvate dehydrogenase kinase isoform 1

• 分子量：
• 49kD

• 背景：
• Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013],

• 功能：
• catalytic activity:ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate.,function:Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.,similarity:Belongs to the PDK/BCKDK protein kinase family.,similarity:Contains 1 histidine kinase domain.,tissue specificity:Expressed predominantly in the heart.,

• 细胞定位：
• Mitochondrion matrix .

• 组织表达：
• Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas.

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