PARP-1 Monoclonal Antibody
- 货号：YM0506
- 应用：WB;FCM;ELISA
- 种属：Human
- 简介：
- >>Base excision repair;>>NF-kappa B signaling pathway;>>Apoptosis;>>Necroptosis;>>Diabetic cardiomyopathy
- 蛋白名称：
- Poly [ADP-ribose] polymerase 1
- 免疫原：
- Synthetic peptide of human PARP-1, conjugated to KLH.
- 特异性：
- PARP-1 Monoclonal Antibody detects endogenous levels of PARP-1 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:500 - 1:2000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- PARP1;ADPRT;PPOL;Poly [ADP-ribose] polymerase 1;PARP-1;ADP-ribosyltransferase diphtheria toxin-like 1;ARTD1;NAD(+) ADP-ribosyltransferase 1;ADPRT 1;Poly[ADP-ribose] synthase 1
- 背景：
- This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008],
- 功能：
- catalytic activity:NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.,function:Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.,miscellaneous:The ADP-D-ribosyl group of NAD(+) is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units.,PTM:Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Poly-ADP-ribosylated by PARP2.,similarity:Contains 1 BRCT
- 细胞定位：
- Nucleus . Nucleus, nucleolus . Chromosome . Localizes to sites of DNA damage. .
- 组织表达：
- Brain,Colon carcinoma,Fibroblast,Lung,Ovarian carcinoma,Skin,
- Western Blot analysis using PARP-1 Monoclonal Antibody against Jurkat (1), K562 (2), HeLa (3), Raji (4),THP-1 (5) and SW620 (6) cell lysate.
- Flow cytometric analysis of Jurkat cells using PARP-1 Monoclonal Antibody (green) and negative control (purple).
