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p27 Monoclonal Antibody

货号：YM0496
应用：WB;ELISA
种属：Human

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靶点：
p27

简介：
>>Endocrine resistance;>>ErbB signaling pathway;>>HIF-1 signaling pathway;>>FoxO signaling pathway;>>Cell cycle;>>PI3K-Akt signaling pathway;>>AGE-RAGE signaling pathway in diabetic complications;>>Cushing syndrome;>>Measles;>>Human papillomavirus infection;>>Epstein-Barr virus infection;>>Pathways in cancer;>>Transcriptional misregulation in cancer;>>Viral carcinogenesis;>>MicroRNAs in cancer;>>Prostate cancer;>>Chronic myeloid leukemia;>>Small cell lung cancer;>>Gastric cancer

基因名称：
CDKN1B

蛋白名称：
Cyclin-dependent kinase inhibitor 1B

Human Gene Id：
1027

Human Swiss Prot No：
P46527

Mouse Swiss Prot No：
P46414

免疫原：
Purified recombinant fragment of human p27 expressed in E. Coli.

特异性：
p27 Monoclonal Antibody detects endogenous levels of p27 protein.

组成：
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

来源：
Monoclonal, Mouse

稀释：
WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

纯化工艺：
Affinity purification

储存：
-15°C to -25°C/1 year(Do not lower than -25°C)

其他名称：
CDKN1B;KIP1;Cyclin-dependent kinase inhibitor 1B;Cyclin-dependent kinase inhibitor p27;p27Kip1

分子量：
22kD

背景：
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014],

功能：
disease:Defects in CDKN1B are the cause of multiple endocrine neoplasia type 4 (MEN4) [MIM:610755]. Multiple endocrine neoplasia (MEN) syndromes are inherited cancer syndromes of the thyroid. MEN4 is a MEN-like syndrome with a phenotypic overlap of both MEN1 and MEN2.,domain:A peptide sequence containing only AA 28-79 retains substantial Kip1 cyclin A/CDK2 inhibitory activity.,function:Important regulator of cell cycle progrssion. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events.,induction:Maximal levels in quiescence cells and early G(1). Levels decrease after mitogen stimulation as cells progress toward S-phase.,miscellaneous:Decreased levels of p27Kip1, mainly due to proteosomal degradation, are found in various epithelial tumors originati

细胞定位：
Nucleus. Cytoplasm. Endosome . Nuclear and cytoplasmic in quiescent cells. AKT- or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89. Colocalizes at the endosome with SNX6; this leads to lysosomal degradation (By similarity). .

组织表达：
Expressed in kidney (at protein level) (PubMed:15509543). Expressed in all tissues tested (PubMed:8033212). Highest levels in skeletal muscle, lowest in liver and kidney (PubMed:8033212).

Long non‑coding RNA ENST00000547547 inhibits cell proliferation, invasion and migration in colorectal cancer cells. ONCOLOGY REPORTS Oncol Rep. 2019 Jan;41(1):483-491 WB Human 1:200 HCT116 cell, LoVo cell
货号：YM0496

Ai, Xiaohui, et al. "Long non-coding RNA ENST00000547547 inhibits cell proliferation, invasion and migration in colorectal cancer cells." Oncology reports 41.1 (2019): 483-491.
货号：YM0496