• 靶点：
• MMP-1

• 简介：
• >>PPAR signaling pathway;>>IL-17 signaling pathway;>>Relaxin signaling pathway;>>Coronavirus disease - COVID-19;>>Pathways in cancer;>>Bladder cancer;>>Rheumatoid arthritis;>>Lipid and atherosclerosis

• 基因名称：
• MMP1

• 蛋白名称：
• Interstitial collagenase

• Human Gene Id：
• 4312

• Human Swiss Prot No：
• P03956

• 免疫原：
• Purified recombinant fragment of human MMP-1 expressed in E. Coli.

• 特异性：
• MMP-1 Monoclonal Antibody detects endogenous levels of MMP-1 protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Monoclonal, Mouse

• 稀释：
• WB 1:500 - 1:2000. IHC 1:200 - 1:1000. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.

• 纯化工艺：
• Affinity purification

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• MMP1;CLG;Interstitial collagenase;Fibroblast collagenase;Matrix metalloproteinase-1;MMP-1

• 分子量：
• 54kD

• 背景：
• matrix metallopeptidase 1(MMP1) Homo sapiens This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down the interstitial collagens, including types I, II, and III. The gene is part of a cluster of MMP genes on chromosome 11. Mutations in this gene are associated with chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016],

• 功能：
• catalytic activity:Cleavage of the triple helix of collagen at about three-quarters of the length of the molecule from the N-terminus, at 775-Gly-|-Ile-776 in the alpha-1(I) chain. Cleaves synthetic substrates and alpha-macroglobulins at bonds where P1' is a hydrophobic residue.,cofactor:Binds 2 zinc ions per subunit.,cofactor:Binds 4 calcium ions per subunit.,domain:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.,domain:There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).,enzyme regulation:Can be activated without removal of the activation peptide.,function:Cleaves col

• 细胞定位：
• Secreted, extracellular space, extracellular matrix .

• 组织表达：
• Fibroblast,Ovary,Synovial cell,Synovial membrane,Thyroid,

The Effects of Injectable Platelet-Rich Fibrin and Advanced-Platelet Rich Fibrin on Gingival Fibroblast Cell Vitality, Proliferation, Differentiation. Tissue Engineering and Regenerative Medicine Yin Lihua WB Human 1:1000 human gingival fibroblast cells (HGFCs)
货号：YM0445

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