• 靶点：
• HXK II

• 简介：
• >>Glycolysis / Gluconeogenesis;>>Fructose and mannose metabolism;>>Galactose metabolism;>>Starch and sucrose metabolism;>>Amino sugar and nucleotide sugar metabolism;>>Neomycin, kanamycin and gentamicin biosynthesis;>>Metabolic pathways;>>Carbon metabolism;>>Biosynthesis of nucleotide sugars;>>HIF-1 signaling pathway;>>Insulin signaling pathway;>>Type II diabetes mellitus;>>Carbohydrate digestion and absorption;>>Shigellosis;>>Central carbon metabolism in cancer

• 基因名称：
• HK2

• 蛋白名称：
• Hexokinase-2

• Human Gene Id：
• 3099

• Human Swiss Prot No：
• P52789

• Mouse Swiss Prot No：
• O08528

• 免疫原：
• Purified recombinant fragment of human HXK II expressed in E. Coli.

• 特异性：
• HXK II Monoclonal Antibody detects endogenous levels of HXK II protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Monoclonal, Mouse

• 稀释：
• WB 1:500 - 1:2000. IHC 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000.. IF 1:50-200

• 纯化工艺：
• Affinity purification

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• HK2;Hexokinase-2;Hexokinase type II;HK II;Muscle form hexokinase

• 分子量：
• 102kD

• 背景：
• Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009],

• 功能：
• catalytic activity:ATP + D-hexose = ADP + D-hexose 6-phosphate.,domain:The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.,enzyme regulation:Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.,miscellaneous:In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).,online information:Hexokinase entry,pathway:Carbohydrate metabolism; hexose metabolism.,polymorphism:Although found in NIDDM patients, genetic variations of HK2 do not contribute to the disease.,similarity:Belongs to the hexokinase family.,subcellular location:Its hydrophobic N-terminal sequence may be involved in membrane binding.,subunit:Monomer.,tissue specificity:Predominant hex

• 细胞定位：
• Mitochondrion outer membrane ; Peripheral membrane protein . Cytoplasm, cytosol . The mitochondrial-binding peptide (MBP) region promotes association with the mitochondrial outer membrane (PubMed:29298880). The interaction with the mitochondrial outer membrane via the mitochondrial-binding peptide (MBP) region promotes higher stability of the protein (PubMed:29298880). Release from the mitochondrial outer membrane into the cytosol induces permeability transition pore (PTP) opening and apoptosis (PubMed:18350175). .

• 组织表达：
• Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.

Glycometabolic adaptation mediates the insensitivity of drug-resistant K562/ADM leukaemia cells to adriamycin via the AKT-mTOR/c-Myc signalling pathway. Molecular Medicine Reports Mol Med Rep. 2017 Apr;15(4):1869-1876 WB Human 1:4000 K562/ADM cell
货号：YM0350

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