CD66e Monoclonal Antibody
- 货号：YM0129
- 应用：WB;IF;ELISA
- 种属：Human
- 蛋白名称：
- Carcinoembryonic antigen-related cell adhesion molecule 5
- 免疫原：
- Purified recombinant fragment of human CD66e expressed in E. Coli.
- 特异性：
- CD66e Monoclonal Antibody detects endogenous levels of CD66e protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- CEACAM5;CEA;Carcinoembryonic antigen-related cell adhesion molecule 5;Carcinoembryonic antigen;CEA;Meconium antigen 100;CD antigen CD66e
- 背景：
- This gene encodes a cell surface glycoprotein that represents the founding member of the carcinoembryonic antigen (CEA) family of proteins. The encoded protein is used as a clinical biomarker for gastrointestinal cancers and may promote tumor development through its role as a cell adhesion molecule. Additionally, the encoded protein may regulate differentiation, apoptosis, and cell polarity. This gene is present in a CEA family gene cluster on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015],
- 功能：
- function:Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.,PTM:Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate.,similarity:Belongs to the immunoglobulin superfamily. CEA family.,similarity:Contains 7 Ig-like (immunoglobulin-like) domains.,subunit:Homodimer. Binding of E.coli Dr adhesins leads to dissociation of the homodimer.,tissue specificity:Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.,
- 细胞定位：
- Cell membrane ; Lipid-anchor, GPI-anchor . Apical cell membrane . Cell surface . Localized to the apical glycocalyx surface. .
- 组织表达：
- Expressed in columnar epithelial and goblet cells of the colon (at protein level) (PubMed:10436421). Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
- Western Blot analysis using CD66e Monoclonal Antibody against HEK293 (1) and CEA-hIgGFc transfected HEK293 (2) cell lysate.
- Immunofluorescence analysis of PANC-1 cells using CD66e Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
