Casein Kinase Iα Monoclonal Antibody
- 货号：YM0092
- 应用：WB;IHC;IF;ELISA
- 种属：Human
- 简介：
- >>Wnt signaling pathway;>>Hedgehog signaling pathway;>>Alzheimer disease;>>Pathways of neurodegeneration - multiple diseases;>>Human papillomavirus infection;>>Breast cancer;>>Hepatocellular carcinoma;>>Gastric cancer
- 蛋白名称：
- Casein kinase I isoform alpha
- 免疫原：
- Purified recombinant fragment of Casein Kinase Iα expressed in E. Coli.
- 特异性：
- Casein Kinase Iα Monoclonal Antibody detects endogenous levels of Casein Kinase Iα protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:500 - 1:2000. IHC 1:200 - 1:1000. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- CSNK1A1;Casein kinase I isoform alpha;CKI-alpha;CK1
- 背景：
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 on 'Ser-45'.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Monomer. Interacts with the Axin complex.,
- 功能：
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 on 'Ser-45'.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Monomer. Interacts with the Axin complex.,
- 细胞定位：
- Cytoplasm . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Chromosome, centromere, kinetochore . Nucleus speckle . Cytoplasm, cytoskeleton, cilium basal body . Cytoplasm, cytoskeleton, spindle . Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis. Also recruited to the keratin cytoskeleton (PubMed:23902688). .
- 组织表达：
- Brain,Epithelium,Liver,Lung,Placenta,Skin,
- Western Blot analysis using Casein Kinase Iα Monoclonal Antibody against truncated CK1 recombinant protein.
- Immunohistochemistry analysis of paraffin-embedded human liver tissue (A), colon carcinoma (B), lung carcinoma (C) and esophagus tissue (D), showing membrane localization with DAB staining using Casein Kinase Iα Monoclonal Antibody.
