• 靶点：
• Histone H3

• 简介：
• >>Neutrophil extracellular trap formation;>>Alcoholism;>>Shigellosis;>>Transcriptional misregulation in cancer;>>Systemic lupus erythematosus

• 基因名称：
• HIST1H3A/HIST1H3B/HIST1H3C/HIST1H3D/HIST1H3E/HIST1H3F/HIST1H3G/HIST1H3H/HIST1H3I/HIST1H3J/HIST2H3A/HIST2H3C/HIST2H3D/H3F3A/H3F3B

• 蛋白名称：
• Histone H3.1/Histone H3.2/Histone H3.3

• Human Gene Id：
• 8350/8351/8352/8353/8354/8355/8356/8357/8358/8968/126961/333932/653604/3020/3021

• Human Swiss Prot No：
• P68431/Q71DI3/P84243

• Mouse Gene Id：
• 319152/15077/15078

• Rat Gene Id：
• 291159/100361558

• Rat Swiss Prot No：
• Q6LED0/P84245

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human Histone H3 around the acetylated site of Lys27. AA range:11-60

• 特异性：
• Acetyl-Histone H3 (K27) Polyclonal Antibody detects endogenous levels of Histone H3 protein only when acetylated at K27.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. IP 1:200 ELISA: 1:5000. Not yet tested in other applications.

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• HIST1H3A;H3FA;HIST1H3B;H3FL;HIST1H3C;H3FC;HIST1H3D;H3FB;HIST1H3E;H3FD;HIST1H3F;H3FI;HIST1H3G;H3FH;HIST1H3H;H3FK;HIST1H3I;H3FF;HIST1H3J;H3FJ;Histone H3.1;Histone H3/a;Histone H3/b;Histone H3/c;Histone H3/d;Histone H3;H3k27AC

• 实测条带：
• 17kD

• 背景：
• Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],

• 功能：
• caution:Was originally (PubMed:2587222) thought to originate from mouse.,developmental stage:Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.,function:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.,mass spectrometry:Monoisotopic with N-acetylserine PubMed:16457589,miscellaneous:This histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2).,PTM:Acetylation is generally l

• 细胞定位：
• Nucleus. Chromosome.

• 组织表达：
• Blood,Epithelium,Kidney,Lung,Ovary,Spleen,Uterus,

Comprehensive landscape of epigenetic-dysregulated lncRNAs reveals a profound role of enhancers in carcinogenesis in breast cancer subtypes. Molecular Therapy-Nucleic Acids 2021 Jan 01 WB Human 1:500 MDA-MB-468s
货号：YK0010

Up-regulation of neprilysin mediates the protection of fructo-oligosaccharides against Alzheimer's disease. Food & Function Food Funct. 2020 Jul;11(7):6565-6572 WB Mouse,Human 1:200 Cerebral cortex SH-SY5Ycell
货号：YK0010

Targeting P16INK4A in uterine serous carcinoma through inhibition of histone demethylation. ONCOLOGY REPORTS Oncol Rep. 2019 May;41(5):2667-2678 WB,IHC Human 1:500 Uterine serous carcinoma (USC) tissue AN3CA cell, Hec1A cell, Hec108 cell, Nou-1 cell, EFE-184 cell, ETN-1 cell
货号：YK0010

AtHD2D, A Plant-specific Histone Deacetylase Involved in Abscisic Acid Response and Lateral Root Development JOURNAL OF EXPERIMENTAL BOTANY Zhaofen Han WB Arabidopsis thaliana
货号：YK0010

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