• 靶点：
• Histone H3

• 简介：
• >>Neutrophil extracellular trap formation;>>Alcoholism;>>Shigellosis;>>Transcriptional misregulation in cancer;>>Systemic lupus erythematosus

• 基因名称：
• HIST1H3A/HIST1H3B/HIST1H3C/HIST1H3D/HIST1H3E/HIST1H3F/HIST1H3G/HIST1H3H/HIST1H3I/HIST1H3J/HIST2H3A/HIST2H3C/HIST2H3D/H3F3A/H3F3B

• 蛋白名称：
• Histone H3.1/Histone H3.2/Histone H3.3

• Human Gene Id：
• 8350/8351/8352/8353/8354/8355/8356/8357/8358/8968/126961/333932/653604/3020/3021

• Human Swiss Prot No：
• P68431/Q71DI3/P84243

• Mouse Gene Id：
• 319152/15077/15078

• Rat Gene Id：
• 291159/100361558

• Rat Swiss Prot No：
• Q6LED0/P84245

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human Histone H3 around the acetylated site of Lys9. AA range:3-52

• 特异性：
• Acetyl-Histone H3 (K9) Polyclonal Antibody detects endogenous levels of Histone H3 protein only when acetylated at K9.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500-2000, IHC 1:50-300, IF 1:50-300

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• HIST1H3A;H3FA;HIST1H3B;H3FL;HIST1H3C;H3FC;HIST1H3D;H3FB;HIST1H3E;H3FD;HIST1H3F;H3FI;HIST1H3G;H3FH;HIST1H3H;H3FK;HIST1H3I;H3FF;HIST1H3J;H3FJ;Histone H3.1;Histone H3/a;Histone H3/b;Histone H3/c;Histone H3/d;Histone H3;H3k9AC

• 实测条带：
• 17kD

• 背景：
• Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],

• 功能：
• caution:Was originally (PubMed:2587222) thought to originate from mouse.,developmental stage:Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.,function:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.,mass spectrometry:Monoisotopic with N-acetylserine PubMed:16457589,miscellaneous:This histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2).,PTM:Acetylation is generally l

• 细胞定位：
• Nucleus. Chromosome.

• 组织表达：
• Blood,Epithelium,Kidney,Lung,Ovary,Spleen,Uterus,

Differential gene regulatory plasticity between upper and lower layer cortical excitatory neurons. MOLECULAR AND CELLULAR NEUROSCIENCE 2018 May 23 WB Mouse brain
货号：YK0006

The elevated transcription of ADAM19 by the oncohistone H2BE76K contributes to oncogenic properties in breast cancer. JOURNAL OF BIOLOGICAL CHEMISTRY 2021 Jan;296: WB Human 293T cell,MDA-MB-231 cell
货号：YK0006

As a Histone Deacetylase Inhibitor, γ‐Aminobutyric Acid Upregulates GluR2 Expression: An In Vitro and In Vivo Study. MOLECULAR NUTRITION & FOOD RESEARCH Mol Nutr Food Res. 2019 Sep;63(17):1900001 WB Mouse,Human 1: 200 Cerebral Cortex SH-SY5Y cell, 3T3-L1 Cell
货号：YK0006

Intermittent Fasting Alleviates the Increase of Lipoprotein Lipase Expression in Brain of a Mouse Model of Alzheimer's Disease: Possibly Mediated by β-hydroxybutyrate. Frontiers in Cellular Neuroscience 2018 Jan 17 WB Human,Mouse 1:200,1:200 Cortex SH-SY5Y cell
货号：YK0006

Up-regulation of neprilysin mediates the protection of fructo-oligosaccharides against Alzheimer's disease. Food & Function Food Funct. 2020 Jul;11(7):6565-6572 WB Mouse,Human 1:200 Cerebral cortex SH-SY5Ycell
货号：YK0006

Association between human cytomegalovirus infection and histone acetylation level in various histological types of glioma. Oncology Letters Oncol Lett. 2015 Nov;10(5):2812-2820 IHC Human 1:200 glioblastoma, anaplastic astrocytoma, low-grade gloma ,normal cortex tissues
货号：YK0006

Chen, Li, Zhigang Miao, and Xingshun Xu. "β-hydroxybutyrate alleviates depressive behaviors in mice possibly by increasing the histone3-lysine9-β-hydroxybutyrylation." Biochemical and biophysical research communications 490.2 (2017): 117-122.
货号：YK0006

As an HDAC Inhibitor, GABA Upregulates GluR2 Expression: A Study of In Vitro and In Vivo." Molecular Nutrition & Food Research: 1900001.
货号：YK0006

Differential gene regulatory plasticity between upper and lower layer cortical excitatory neurons. MOLECULAR AND CELLULAR NEUROSCIENCE 2018 May 23 WB Mouse brain
货号：YK0006

The elevated transcription of ADAM19 by the oncohistone H2BE76K contributes to oncogenic properties in breast cancer. JOURNAL OF BIOLOGICAL CHEMISTRY 2021 Jan;296: WB Human 293T cell,MDA-MB-231 cell
货号：YK0006

Glycochenodeoxycholate Affects Iron Homeostasis via Up-Regulating Hepcidin Expression Nutrients Long-jiao Wang WB Human,Mouse
货号：YK0006

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