DDDDK-Tag(binds to flag sequnence) rabbit pAb
- 货号:YG0004
- 应用:WB;ELISA;IP;IF
- 种属:Species independent
- 靶点:
- FLAG-tag
- 基因名称:
- Flag tag; Flag-tag,DDDDK TAG, DDDDK-TAG, DYKDDDDK tag,DYKDDDDK-tag
- 免疫原:
- DDDDK synthetic peptide conjugated to KLH.
- 特异性:
- FLAG-tag Polyclonal Antibody detects FLAG-tagged recombinant proteins or FLAG-tagged proteins overexpressed in cells.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:1000 - 1:3000. ELISA: 1:5000-20000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 浓度:
- 1 mg/ml
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- DDDDK epitope tag;DDDDK epitope tag;DYKDDDDK epitope tag
- 背景:
- The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.
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- 产品图片
- Western Blot analysis using FLAG-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing FLAG tag (1) and untransfected (2). Secondary antibody(catalog#:RS0002) was diluted at 1:20000