• 靶点：
• PARP

• 简介：
• >>Base excision repair;>>NF-kappa B signaling pathway;>>Apoptosis;>>Necroptosis;>>Diabetic cardiomyopathy

• 基因名称：
• PARP1

• 蛋白名称：
• Poly [ADP-ribose] polymerase 1

• Human Gene Id：
• 142

• Human Swiss Prot No：
• P09874

• Mouse Swiss Prot No：
• P11103

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human PARP. AA range:196-245

• 特异性：
• Cleaved-PARP-1 (G215) Polyclonal Antibody detects endogenous levels of fragment of activated PARP-1 protein resulting from cleavage adjacent to G215.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• PARP1;ADPRT;PPOL;Poly [ADP-ribose] polymerase 1;PARP-1;ADP-ribosyltransferase diphtheria toxin-like 1;ARTD1;NAD(+) ADP-ribosyltransferase 1;ADPRT 1;Poly[ADP-ribose] synthase 1

• 实测条带：
• 89kD

• 背景：
• This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008],

• 功能：
• catalytic activity:NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.,function:Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.,miscellaneous:The ADP-D-ribosyl group of NAD(+) is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units.,PTM:Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Poly-ADP-ribosylated by PARP2.,similarity:Contains 1 BRCT

• 细胞定位：
• Nucleus . Nucleus, nucleolus . Chromosome . Localizes to sites of DNA damage. .

• 组织表达：
• Brain,Colon carcinoma,Fibroblast,Lung,Ovarian carcinoma,Skin,

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Loss of Tctn3 causes neuronal apoptosis and neural tube defects in mice. Cell Death & Disease Cell Death Dis. 2018 May;9(5):1-12 WB Mouse 1:100 brain
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Exogenous Adipokine Peptide Resistin Protects Against Focal Cerebral Ischemia/Reperfusion Injury in Mice. NEUROCHEMICAL RESEARCH Neurochem Res. 2017 Oct;42(10):2949-2957 WB Mouse right hemisphere
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RNAi-mediated knockdown of the CLN3 gene inhibits proliferation and promotes apoptosis in drug-resistant ovarian cancer cells. Molecular Medicine Reports 2015 Nov 01 WB Human 1:1000 A2780 cell
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Sodium butyrate induces autophagic apoptosis of nasopharyngeal carcinoma cells by inhibiting AKT/mTOR signaling. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Biochem Bioph Res Co. 2019 Jun;514:64 WB Human 5–8F cell, HK-1 cell
货号：YC0073

Shikonin potentiates therapeutic efficacy of oxaliplatin through reactive oxygen species-mediated intrinsic apoptosis and endoplasmic reticulum stress in oxaliplatin-resistant colorectal cancer cells DRUG DEVELOPMENT RESEARCH Yajie Zhang WB Human,Mouse HCT116R-xenograft OXA-resistant CRC cells (HCT116R)
货号：YC0073

EDIL3/Del-1 prevents aortic dissection through enhancing internalization and degradation of apoptotic vascular smooth muscle cells Autophagy Zheng Yin WB Mouse 1:1000 Aortic tissue macrophages
货号：YC0073

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