• 靶点：
• Caspase-9

• 简介：
• >>Platinum drug resistance;>>p53 signaling pathway;>>PI3K-Akt signaling pathway;>>Apoptosis;>>Apoptosis - multiple species;>>VEGF signaling pathway;>>Thyroid hormone signaling pathway;>>Alzheimer disease;>>Parkinson disease;>>Amyotrophic lateral sclerosis;>>Huntington disease;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Pathogenic Escherichia coli infection;>>Legionellosis;>>Toxoplasmosis;>>Tuberculosis;>>Hepatitis C;>>Hepatitis B;>>Measles;>>Human cytomegalovirus infection;>>Influenza A;>>Kaposi sarcoma-associated herpesvirus infection;>>Herpes simplex virus 1 infection;>>Epstein-Barr virus infection;>>Human immunodeficiency virus 1 infection;>>Pathways in cancer;>>Colorectal cancer;>>Pancreatic cancer;>>Endometrial cancer;>>Prostate cancer;>>Small cell lung cancer;>>Non-small cell lung cancer;>>Viral myocarditis;>>Lipid and atherosclerosis

• 基因名称：
• CASP9

• 蛋白名称：
• Caspase9

• Human Gene Id：
• 842

• Human Swiss Prot No：
• P55211

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human Caspase 9. AA range:281-330

• 特异性：
• Cleaved-Caspase-9 p10 (D330) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-9 p10 protein resulting from cleavage adjacent to D330.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000. Not yet tested in other applications.

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• CASP9;MCH6;Caspase-9;CASP-9;Apoptotic protease Mch-6;Apoptotic protease-activating factor 3;APAF-3;ICE-like apoptotic protease 6;ICE-LAP6

• 实测条带：
• 10 46kD

• 背景：
• CASP9 encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Caspase 9 can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. Caspase 9 is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.

• 功能：
• catalytic activity:Strict requirement for an Asp residue at position P1 and with a marked preference for His at position P2. It has a preferred cleavage sequence of Leu-Gly-His-Asp-|-Xaa.,function:Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).,function:Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.,online information:Caspase-9 entry,PTM:Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.,similarity:Belongs to the peptidase C14A family.,similarity:Contains 1 CARD domain.,subunit:Heterotetramer that consists of two anti-parallel arranged heterodimers

• 细胞定位：
• nucleus,mitochondrion,cytosol,apoptosome,

• 组织表达：
• Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.

Nuclear factor Nrf2 promotes glycosidase OGG1 expression by activating the AKT pathway to enhance leukemia cell resistance to cytarabine JOURNAL OF BIOLOGICAL CHEMISTRY Qin Shang, Chengyun Pan, Xi Zhang, Tonghua Yang, Tianzhen Hu, Lin Zheng, Shuyun Cao, Cheng Feng, Xiuying Hu, Xiao Chai, Jishi Wang, Qin Fang WB Human U937 cell, THP-1 cell
货号：YC0013

Targeting FoxM1 by thiostrepton inhibits growth and induces apoptosis of laryngeal squamous cell carcinoma. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY 2014 Nov 13 WB Human Hep-2 cell
货号：YC0013

Ginsenoside Rg5 induces apoptosis in human esophageal cancer cells through the phosphoinositide‑3 kinase/protein kinase B signaling pathway. Molecular Medicine Reports Mol Med Rep. 2019 May;19(5):4019-4026 WB Human 1:1000 Eca-109 cell
货号：YC0013

Promotion of tumor progression induced by continuous low-dose administration of antineoplastic agent gemcitabine or gemcitabine combined with cisplatin LIFE SCIENCES Weiyi Feng WB Human
货号：YC0013

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