GST-Tag(PNF002) Nb-FC recombinant antibody
- 货号：YA0649
- 应用：WB
- 种属：Species independent
- 免疫原：
- Recombinant Protein of GST-Tag
- 特异性：
- The antibody detects GST tag fusion proteins.
- 组成：
- Phosphate-buffered solution
- 来源：
- Camel, chimeric fusion of Nanobody (VHH) and mouse IgG1 Fc domain , recombinantly produced from 293F cell
- 纯化工艺：
- Recombinant Expression and Affinity purified
- 储存：
- -15°C to -25°C/1 year(Avoid freeze / thaw cycles)
- 背景：
- The glutathione S-transferase (GST, previously known as ligandins) family of enzymes are composed of many cytosolic, mitochondrial, and microsomal proteins. GSTs are present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions and accept endogenous and xenobiotic substrates. GST tag antibody can be helpful in detecting the fusion protein during purification as well as the cleavage of GST from the protein of interest.GST tag antibody has wide applications that could include your research on GST proteins or GST fusion recombinant proteins.
- Whole extracts were separated by 12% SDS-PAGE, and the membrane was blotted with anti-GST-Tag(PNF002)antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody.
Lane 1: BL21 transfected with an empty expression vector
Lane 2: BL21 transfected with a GST-Tag protein
Predicted band size: 26kDa
Observed band size: 26kDa
