Cleaved-TACE (R215) Cell-Based Colorimetric ELISA Kit
- 货号：KA3931C
- 应用：ELISA
- 种属：Human
- 其他名称：
- Disintegrin and metalloproteinase domain-containing protein 17 (ADAM 17) (EC 3.4.24.86) (Snake venom-like protease) (TNF-alpha convertase) (TNF-alpha-converting enzyme) (CD antigen CD156b)
- 背景：
- catalytic activity:Narrow endopeptidase specificity. Cleaves Pro-Leu-Ala-Gln-Ala-|-Val-Arg-Ser-Ser-Ser in the membrane-bound, 26-kDa form of tumor necrosis factor alpha (TNF-alpha). Similarly cleaves other membrane-anchored, cell-surface proteins to 'shed' the extracellular domains.,cofactor:Binds 1 zinc ion per subunit.,domain:Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.,domain:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.,function:Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway.,induction:In arthritis-affected cartilage.,online information:Tumor necrosis factor alpha-converting enzyme entry,PTM:Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791.,PTM:The precursor is cleaved by a furin endopeptidase.,similarity:Contains 1 disintegrin domain.,similarity:Contains 1 peptidase M12B domain.,subunit:Interacts with MAD2L1 and MUC1.,tissue specificity:Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.,
- 功能：
- regulation of cyclin-dependent protein kinase activity, G1/S transition of mitotic cell cycle, mitotic cell cycle, regulation of cell growth, response to hypoxia, cell activation, regulation of cytokine production, negative regulation of cytokine production, positive regulation of cytokine production, regulation of protein amino acid phosphorylation, positive regulation of protein amino acid phosphorylation, response to molecule of bacterial origin, adaptive immune response,immune effector process, leukocyte mediated immunity, myeloid leukocyte mediated immunity, neutrophil mediated immunity, adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains, germinal center formation, immune system development, leukocyte differentiation, positive regulation of immune system process, regulation of leukocyte migration, positive regula
- 细胞定位：
- Membrane; Single-pass type I membrane protein.
- 组织表达：
- Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney. Expressed in natural killer cells (at protein level) (PubMed:24337742).
