Phospho HSF1 (S121) Cell-Based Colorimetric ELISA Kit

  • 货号：KA1685C
  • 应用：ELISA
  • 种属：Human;Mouse
    • 基因名称：
    • HSF1
    • Human Gene Id：
    • 3297
    • Human Swiss Prot No：
    • Q00613
    • Mouse Swiss Prot No：
    • P38532
    • 储存：
    • 2-8°C/6 months
    • 其他名称：
    • Heat shock factor protein 1 (HSF 1) (Heat shock transcription factor 1) (HSTF 1)
    • 检测方法：
    • Colorimetric
    • 背景：
    • function:DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.,PTM:Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat.,PTM:Sumoylated BY SUMO1 AND SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.,similarity:Belongs to the HSF family.,subcellular location:Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.,subunit:Monomer. Under normal conditions, interacts with HSP90AA1 in the HSP90 multichaperone complex; the interaction prevents trimerization and activation of HSF1. On activation by heat-stress or by other factors such as metal ions, HSF1 is released from the complex, homotrimerizes, is hyperphosphorylated and translocated to the nucleus where, subsequently, it can activate transcription. Binds the complex through the regulatory domain. Interacts with SYMPK and CSTF2 in heat-stressed cells. Interacts with FKBP4 in the HSP90 multichaperone complex; the interaction is independent of the phosphorylation state of HSF1. Interacts with MAPKAPK2.,
    • 功能：
    • in utero embryonic development, regulation of cytokine production, negative regulation of cytokine production,placenta development, embryonic placenta development, response to molecule of bacterial origin, transcription,regulation of transcription, DNA-dependent, protein amino acid phosphorylation, phosphorus metabolic process,phosphate metabolic process, defense response, gamete generation, spermatogenesis, female pregnancy, negative regulation of cell proliferation, response to temperature stimulus, response to heat, response to bacterium, response to abiotic stimulus, embryonic development ending in birth or egg hatching, response to organic substance,phosphorylation, sexual reproduction, response to lipopolysaccharide, multicellular organism reproduction, regulation of tumor necrosis factor production, negative regulation of tumor necrosis factor production, regulation of growth,regu
    • 细胞定位：
    • Nucleus . Cytoplasm . Nucleus, nucleoplasm . Cytoplasm, perinuclear region . Cytoplasm, cytoskeleton, spindle pole . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Chromosome, centromere, kinetochore . The monomeric form is cytoplasmic in unstressed cells (PubMed:8455624, PubMed:26159920). Predominantly nuclear protein in both unstressed and heat shocked cells (PubMed:10413683, PubMed:10359787). Translocates in the nucleus upon heat shock (PubMed:8455624). Nucleocytoplasmic shuttling protein (PubMed:26159920). Colocalizes with IER5 in the nucleus (PubMed:27354066). Colocalizes with BAG3 to the nucleus upon heat stress (PubMed:8455624, PubMed:26159920). Localizes in subnuclear granules called nuclear stress bodies (nSBs) upon heat shock (PubMed:11447121, PubMed:1151455

    相关报价

    • 货号：
    • KA1685C
    • 规格：
    • 96 / well
    • 价格：
    • ¥4700
    • 货期：
    • 预计发货时间为
      2024-08-01
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