Synaptotagmin 1/2 (phospho Ser309/306) Polyclonal Antibody
- Catalog No.：YP0586
- Applications：WB;IHC;IF;ELISA
- Reactivity：Human;Mouse;Rat
- Target：
- Synaptotagmin 1/2
- Fields：
- >>Synaptic vesicle cycle
- Gene Name：
- SYT1/SYT2
- Protein Name：
- Synaptotagmin-1/2
- Human Gene Id：
- 6857/127833
- Human Swiss Prot No：
- P21579/Q8N9I0
- Mouse Gene Id：
- 20979/20980
- Rat Gene Id：
- 25716/24805
- Rat Swiss Prot No：
- P21707/P29101
- Immunogen：
- The antiserum was produced against synthesized peptide derived from human Synaptotagmin around the phosphorylation site of Ser309. AA range:276-325
- Specificity：
- Phospho-Synaptotagmin 1/2 (S309/306) Polyclonal Antibody detects endogenous levels of Synaptotagmin 1/2 protein only when phosphorylated at S309/306.
- Formulation：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source：
- Polyclonal, Rabbit,IgG
- Dilution：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- Purification：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration：
- 1 mg/ml
- Storage Stability：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name：
- SYT1;SVP65;SYT;Synaptotagmin-1;Synaptotagmin I;SytI;p65;SYT2;Synaptotagmin-2;Synaptotagmin II;SytII
- Observed Band(KD)：
- 47kD
- Background：
- The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010],
- Function：
- cofactor:Binds 3 calcium ions per subunit. The ions are bound to the C2 domains.,domain:The first C2 domain mediates Ca(2+)-dependent phospholipid binding.,domain:The second C2 domain mediates interaction with SV2A and STN2.,function:May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.,similarity:Belongs to the synaptotagmin family.,similarity:Contains 2 C2 domains.,subcellular location:Synaptic vesicles and chromaffin granules.,subunit:H
- Subcellular Location：
- Cytoplasmic vesicle, secretory vesicle membrane ; Single-pass membrane protein . Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane ; Single-pass membrane protein . Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane ; Single-pass membrane protein . Cytoplasm .
- Expression：
- Expressed in melanocytes (PubMed:23999003).
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- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using Phospho-Synaptotagmin 1/2 (S309/306) Polyclonal Antibody
- Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Synaptotagmin (Phospho-Ser309) Antibody
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Synaptotagmin (Phospho-Ser309) Antibody. The picture on the right is blocked with the phospho peptide.
- Western blot analysis of lysates from 293 cells treated with Sobital 0.4M 30', using Synaptotagmin (Phospho-Ser309) Antibody. The lane on the right is blocked with the phospho peptide.
