• Target：
• Smad1

• Fields：
• >>TGF-beta signaling pathway;>>Hippo signaling pathway;>>Signaling pathways regulating pluripotency of stem cells;>>Transcriptional misregulation in cancer

• Gene Name：
• SMAD1

• Protein Name：
• Mothers against decapentaplegic homolog 1

• Human Gene Id：
• 4086

• Human Swiss Prot No：
• Q15797

• Mouse Gene Id：
• 17125

• Mouse Swiss Prot No：
• P70340

• Rat Swiss Prot No：
• P97588

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human Smad1 around the phosphorylation site of Ser465. AA range:416-465

• Specificity：
• Phospho-Smad1 (S465) Polyclonal Antibody detects endogenous levels of Smad1 protein only when phosphorylated at S465.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• SMAD1;BSP1;MADH1;MADR1;Mothers against decapentaplegic homolog 1;MAD homolog 1;Mothers against DPP homolog 1;JV4-1;Mad-related protein 1;SMAD family member 1;SMAD 1;Smad1;hSMAD1;Transforming growth factor-beta-signaling protein

• Observed Band(KD)：
• 60kD

• Background：
• The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-med

• Function：
• function:Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD).,PTM:Phosphorylated on serine by BMP type 1 receptor kinase.,PTM:Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.,similarity:Belongs to the dwarfin/SMAD family.,similarity:Contains 1 MH1 (MAD homology 1) domain.,similarity:Contains 1 MH2 (MAD homology 2) domain.,subcellular location:Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.,subunit:Interacts with HGS, NANOG and ZCCHC12 (By similarity). May form trimers with another SMAD1 and the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit, CREB-binding protein (CBP), p300, SMURF1, SMURF2 and HOXC8. Associates with ZNF423 or ZNF521 in response to BMP2 leading to activate transcription of BMP target genes. Interacts with LBXCOR1.,

• Subcellular Location：
• Cytoplasm . Nucleus . Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4 (PubMed:15647271). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15647271). Exported from the nucleus to the cytoplasm when dephosphorylated (By similarity). .

• Expression：
• Ubiquitous. Highest expression seen in the heart and skeletal muscle.

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