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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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PRD16 rabbit pAb
- 货号:YT8124
- 应用:IHC;WB
- 种属:Human;Mouse
- 基因名称:
- PRDM16 KIAA1675 MEL1 PFM13
- 蛋白名称:
- PR domain zinc finger protein 16 (PR domain-containing protein 16) (Transcription factor MEL1) (MDS1/EVI1-like gene 1)
- 免疫原:
- Synthesized peptide derived from human C-ternal PRD16
- 特异性:
- This antibody detects endogenous levels of PRD16 at Human, Mouse
- 组成:
- Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000 IHC 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- PR domain zinc finger protein 16 (PR domain-containing protein 16) (Transcription factor MEL1) (MDS1/EVI1-like gene 1)
- 功能:
- Binds DNA and functions as a transcriptional regulator . Displays histone methyltransferase activity and monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro (By similarity). Probably catalyzes the monomethylation of free histone H3 in the cytoplasm which is then transported to the nucleus and incorporated into nucleosomes where SUV39H methyltransferases use it as a substrate to catalyze histone H3 'Lys-9' trimethylation (By similarity). Likely to be one of the primary histone methyltransferases along with MECOM/PRDM3 that direct cytoplasmic H3K9me1 methylation (By similarity). Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism (By similarity). To
- 细胞定位:
- Nucleus . Cytoplasm .
- 组织表达:
- Expressed in uterus and kidney. Expressed in both cardiomyocytes and interstitial cells.