• 靶点：
• ATG5

• 简介：
• >>Autophagy - other;>>Mitophagy - animal;>>Autophagy - animal;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Ferroptosis;>>NOD-like receptor signaling pathway;>>RIG-I-like receptor signaling pathway;>>Shigellosis

• 基因名称：
• ATG5 APG5L ASP

• 蛋白名称：
• ATG5

• Human Gene Id：
• 9474

• Human Swiss Prot No：
• Q9H1Y0

• Mouse Gene Id：
• 11793

• Mouse Swiss Prot No：
• Q99J83

• Rat Swiss Prot No：
• Q3MQ06

• 免疫原：
• Synthesized peptide derived from human ATG5 AA range: 180-260

• 特异性：
• This antibody detects endogenous levels of Human,Mouse,Rat ATG5

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:1000-2000 ELISA 1:5000-20000

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• Autophagy protein 5 (APG5-like;Apoptosis-specific protein)

• 分子量：
• 30kD

• 实测条带：
• 51kD

• 背景：
• The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015],

• 功能：
• function:May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.,function:Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed.,induction:By apoptotic stimuli.,PTM:Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.,similarity:Belongs to the ATG5 family.,subcellular location:Colocalizes with nonmuscle actin.,tissue specificity:Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.,

• 细胞定位：
• Cytoplasm . Preautophagosomal structure membrane; Peripheral membrane protein. Colocalizes with nonmuscle actin. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme. .

• 组织表达：
• Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.

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